Isolation, Culture, and Adipogenic Induction of Stromal Vascular Fraction-derived Preadipocytes from Mouse Periaortic Adipose Tissue

Summary

Here, we describe the isolation, culture, and adipogenic induction of stromal vascular fraction-derived preadipocytes from mouse periaortic adipose tissue, allowing for the study of perivascular adipose tissue function and its relationship with vascular cells.

Abstract

Perivascular adipose tissue (PVAT) is an adipose tissue depot that surrounds blood vessels and exhibits the phenotypes of white, beige, and brown adipocytes. Recent discoveries have shed light on the central role of PVAT in regulating vascular homeostasis and participating in the pathogenesis of cardiovascular diseases. A comprehensive understanding of PVAT properties and regulation is of great importance for the development of future therapies. Primary cultures of periaortic adipocytes are valuable for studying PVAT function and the crosstalk between periaortic adipocytes and vascular cells. This paper presents an economical and feasible protocol for the isolation, culture, and adipogenic induction of stromal vascular fraction-derived preadipocytes from mouse periaortic adipose tissue, which can be useful for modeling adipogenesis or lipogenesis in vitro. The protocol outlines tissue processing and cell differentiation for culturing periaortic adipocytes from young mice. This protocol will provide the technological cornerstone at the bench side for the investigation of PVAT function.

Introduction

Perivascular adipose tissue (PVAT), a perivascular structure composed of a mixture of mature adipocytes and a stromal vascular fraction (SVF), is believed to interact with the adjacent vessel wall via its secretome paracrineally¹. As a critical regulator of vascular homeostasis, PVAT dysfunction is implicated in the pathogenesis of cardiovascular diseases^2,3,4. The SVF of adipocyte tissue consists of several expected cell populations, including endothelial cells, immune cells, mesothelium cells, neuronal cells, and adipose stem and progenitor cells (ASPCs)^5,6. It is well known that ASPCs residing in the SVF of adipose tissue can give rise to mature adipocytes⁵. SVF is inferred to be a critical source of mature adipocytes in PVAT. Several studies have shown that PVAT-SVF can differentiate into mature adipocytes under specific induction conditions^6,7,8.

Currently, there are two isolation systems for isolating SVF from adipose tissue, one is enzymatic digestion and the other is non-enzymatic⁹. Enzymatic methods typically result in a higher yield of nucleated progenitor cells¹⁰. To date, the benefits of SVF in promoting vascular regeneration and neovascularization in wound healing, urogenital, and cardiovascular diseases have been widely demonstrated¹¹, especially in dermatology and plastic surgery^12,13. However, the clinical application prospects of PVAT-derived SVF have not been well explored, which may be attributed to the lack of a standardized method for the isolation of SVF from PVAT. The objective of this protocol is to establish a standardized approach for the isolation, culture, and adipogenic induction of SVF-derived preadipocytes from mouse PVAT surrounding the thoracic aorta, enabling further investigation of PVAT function. This protocol optimizes tissue processing and cell differentiation techniques for culturing periaortic adipocytes obtained from young mice.

Protocol

The animal protocols were approved by the Institutional Animal Care and Use Committee at Shanghai Chest Hospital affiliated to Shanghai Jiao Tong University School of Medicine (approval number: KS23010) and were in compliance with relevant ethical regulations. Male and female C57BL/6 mice aged 4-8 weeks are to be preferred for this experiment.

1. Preparation of surgical tools, buffers, and culture media

1. Autoclave surgical tools (e.g., surgical scissors and standard forceps) at 121 °C for 30 min. Disinfect microsurgical instruments with 75% alcohol.
2. Prepare sterile phosphate-buffered saline (PBS) supplemented with 1% v/v penicillin-streptomycin and another 10 mL of PBS supplemented with 10% v/v penicillin-streptomycin.
   NOTE: In the following sections, if not specifically mentioned, PBS refers to regular sterile PBS.
3. Prepare Krebs Ringer HEPES BSA buffer: 1% bovine serum albumin (BSA), 20 mM HEPES dissolved in Krebs Ringer solution.
4. Prepare fresh digestion solution: type 1 collagenase (1 mg/mL) and dispase II (4 mg/mL) dissolved in Krebs Ringer HEPES BSA buffer. Sterilize the solution using a 0.2 µm filter.
5. Prepare a collagen-coated 12-well plate.
   1. Dilute the collagen solution (1 mg/mL) with sterile deionized water to the concentration of 40 µg/mL. Add 1 mL of the diluted collagen solution on the surface of each well to achieve a concentration of 6-10 µg/cm². Incubate the coating for 1 h at room temperature.
   2. Remove the remaining solution and rinse each well 2x with 1 mL of PBS. Use the plate immediately, or air dry the plate in a class II laminar flow hood and store the coated plate at 2-8 °C. When storing, seal the coated plates with parafilm.
6. Prepare culture medium: high-glucose Dulbecco's-Modified Eagles Medium (DMEM), 10% fetal bovine serum (FBS), 1% v/v penicillin-streptomycin. Keep at 4 °C for up to 2 weeks.
7. Prepare lipogenic induction medium: high-glucose DMEM, 10% FBS, 1% v/v penicillin-streptomycin, 1 nM triiodothyronine, 1 µM rosiglitazone, 1 µM insulin, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), and 1 µM dexamethasone. Keep at 4 °C for up to 2 weeks.
8. Prepare maintenance medium: high-glucose DMEM, 10% FBS, 1% v/v penicillin-streptomycin, 1 nM triiodothyronine, 1 µM rosiglitazone, and 1 µM insulin. Keep at 4 °C for up to 2 weeks.

2. Dissection and isolation of perivascular adipose tissue (PVAT)

1. Euthanize the mouse by cervical dislocation under 2% isoflurane anesthesia or with carbon dioxide overdose. Spray with 75% alcohol for skin disinfection.
2. Place the mouse in a supine position (facing upward).
3. Lift the skin, make a small incision, and bluntly separate the skin and abdominal muscles with surgical scissors along the ventral midline from the pelvis to the neck. Expose the heart and lungs by cutting the diaphragm and ribs along both sides of the midline.
4. Carefully remove the liver, spleen, bowels, and kidney. Avoid cutting the intestinal wall.
5. Remove the lungs and esophagus.
6. Gently lift the heart with forceps in one hand and separate the aorta from the spine with surgical scissors in the other hand. Then, place the heart and aorta in a 60 mm Petri dish with PBS containing 1% v/v penicillin-streptomycin.
7. Remove microsurgical forceps and microsurgical scissors from alcohol and rinse in 25 mL of PBS to remove the excess alcohol. Under a stereo microscope, remove the thymus and other tissue from the heart and aorta, then remove the heart, leaving the aorta with the PVATs.
8. Transfer the aorta with the PVATs to a clean 60 mm Petri dish with PBS containing 1% v/v penicillin-streptomycin.
9. Use microsurgical forceps to strip PVATs, with one pair of forceps fixing the aorta and the other pulling off the adipose tissue. Remove the vasculature tissue as much as possible while minimizing damage to the perivascular adipose tissue.
10. Collect the PVAT surrounding the thoracic aorta into the 2 mL microcentrifuge tube containing high-glucose DMEM supplemented with 1% v/v penicillin-streptomycin placed on ice.

3. Isolation of stromal vascular fraction (SVF)

1. Rinse the collected tissues sequentially with PBS containing 10% v/v penicillin-streptomycin and PBS containing 1% v/v penicillin-streptomycin.
   NOTE: From this step onwards, all experiments must be carried out under sterile conditions in a class II laminar flow hood.
2. Transfer the tissues to a sterile 60 mm Petri dish, add 200 µL of digestion solution, and mince them into 1 mm³ pieces using sterile scissors.
3. Transfer the mix to a 15 mL centrifuge tube using a plastic pipette tip, the end broadened by a sterile scissor cut, and add 6 mL of digestion solution to start the digestion reaction.
   NOTE: One digestion reaction (3 mL of digestion solution) is sufficient for PVAT depots from six mice.
4. Incubate the tissues at 37 °C in an incubator with an orbital shaker (150 rpm frequency for effective mixing) for 30-45 min. Tie the tubes flat on a rack to make the tubes shake horizontally. Shake up and down vigorously by hand for 10 s every 5-10 min.
   NOTE: Digestion can be discontinued when a homogeneous, yellowish digestive fluid is seen with no tissue fragments left.
5. Strain the digested tissues through a 70 µm cell strainer into a 50 mL centrifuge tube. Rinse the cell strainer with an equal volume of culture medium to maximize cell yield and stop the digestion.
6. Transfer the filtrate to a new 15 mL centrifuge tube and centrifuge at 1,800 × g for 10 min. Invert the tube to discard the supernatant and resuspend the pellets in 5 mL of PBS. Centrifuge at 1,800 × g for 5 min.
7. Discard the supernatant by inverting the tube and resuspend the pellets in an appropriate volume of culture medium.
   NOTE: The cell pellets collected from every six mice are resuspended in 1 mL of culture medium and seeded into one well of a 12-well plate.
8. Seed the cells into a collagen-coated 12-well plate and incubate at 37 °C in a humid atmosphere with 5% CO₂ overnight.
9. On the next day, aspirate the culture medium, wash the cells with prewarmed (37 °C) PBS containing 1% v/v penicillin-streptomycin to remove cell debris and red blood cells, and add back 1 mL of fresh culture medium each well.

4. Adipogenic induction of SVF-derived preadipocytes from periaortic adipose tissue

1. Change the culture medium every other day until the cells reach ~60-70% confluence.
   NOTE: It usually takes 3-4 days for the cells to reach 60-70% confluence.
2. When the cells reach 60-70% confluence, aspirate the culture medium and replace it with brown adipogenic induction medium. Consider the day of induction of adipogenic differentiation as day 0 of differentiation.
3. After 72 h (day 3 of differentiation), refresh the medium with maintenance medium. Change the maintenance medium every 2 days until the cells are used for experiments.
4. Analyze the adipogenic cells in an appropriate way, such as Oil Red O staining¹⁴ and western blotting¹⁵.

Results

Using this protocol described above, we carefully isolated PVATs surrounding mouse thoracic aortas (Figure 1A-D). After washing and mincing the PVATs into small pieces using sterile scissors (Figure 1E,F), tissue fragments were digested in a digestion solution containing type 1 collagenase (1 mg/mL) and dispase II (4 mg/mL) and incubated at 37 °C on a shaker for 30-45 min (Figure 1G

Discussion

We propose a practical and feasible approach for the isolation and adipogenic induction of SVF-derived preadipocytes from mouse periaortic adipose tissue. The advantages of this protocol are that it is simple and economical. Adequate numbers of mice are critical for successful isolation, as insufficient tissue can result in low SVF density and poor growth state, ultimately affecting lipogenic efficiency. Additionally, mouse age is an important factor to consider as the adipogenic potential of SVF decreases with age. Rapi...

Materials

Name	Company	Catalog Number	Comments
0.2 μm syringe filters	PALL	4612
12-well plate	Labselect	11210
15 mL centrifuge tube	Labserv	310109003
3,3',5-triiodo-L-thyronine (T3)	Sigma-Aldrich	T-2877	1 nM
50 mL centrifuge tube	Labselect	CT-002-50A
anti-adiponectin	Abcam	ab22554	1:1,000 working concentration
anti-COX IV	CST	4850	1:1,000 working concentration
anti-FABP4	CST	2120	1:1,000 working concentration
anti-PGC1α	Abcam	ab191838	1:1,000 working concentration
anti-PPARγ	Invitrogen	MA5-14889	1:1,000 working concentration
anti-UCP1	Abcam	ab10983	1:1,000 working concentration
anti-α-Actinin	CST	6487	1:1,000 working concentration
BSA	Beyotime	ST023-200g	1%
C57BL/6 mice aged 4-8 weeks of both sexes	Shanghai Model Organisms Center, Inc.
Cell Strainer 70 µm, nylon	Falcon	352350
Collagen from calf skin	Sigma-Aldrich	C8919
Collagenase, Type 1	Worthington	LS004196	1 mg/mL
Dexamethasone	Sigma-Aldrich	D1756	1 μM
Dispase II	Sigma-Aldrich	D4693-1G	4 mg/mL
Fetal bovine serum	Gibco	16000-044	10%
HEPES	Sigma-Aldrich	H4034-25G	20 mM
High glucose DMEM	Hyclone	SH30022.01
IBMX	Sigma-Aldrich	I7018	0.5 mM
Incubator with orbital shaker	Shanghai longyue Instrument Eruipment Co.,Ltd.	LYZ-103B
Insulin (cattle)	Sigma-Aldrich	11070-73-8	1 μM
Isoflurane	RWD	R510-22-10
Krebs-Ringer's Solution	Pricella	PB180347	protect from light
Microsurgical forceps	Beyotime	FS233
Microsurgical scissor	Beyotime	FS217
Oil Red O	Sangon Biotech (Shanghai) Co., Ltd	A600395-0050
PBS (Phosphate-buffered saline)	Sangon Biotech (Shanghai) Co., Ltd	B548117-0500
Penicillin-Streptomycin	Gibco	15140122
Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)	Jackson ImmunoResearch	115-035-146	1:5,000 working concentration
Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)	Jackson ImmunoResearch	111-035-144	1:5,000 working concentration
Rosiglitazone	Sigma-Aldrich	R2408	1 μM
Standard forceps	Beyotime	FS225
Surgical scissor	Beyotime	FS001