Nanoparticle Delivery of an Oligonucleotide Payload in a Glioblastoma Multiforme Animal Model

Summary

The blood-brain barrier is a significant hurdle in the delivery of therapies for glioblastoma, a disease for which there is no cure. Here, we report an in vivo image-guided iron oxide therapeutic nano platform that can bypass this physiological barrier by virtue of size and accumulate in the tumor.

Abstract

Glioblastoma multiforme (GBM) is the most common and aggressive form of primary brain malignancy for which there is no cure. The blood-brain barrier is a significant hurdle in the delivery of therapies to GBM. Reported here is an image-guided, iron oxide-based therapeutic delivery nano platform capable of bypassing this physiological barrier by virtue of size and accumulating in the tumor region, delivering its payload. This 25 nm nano platform consists of crosslinked dextran-coated iron oxide nanoparticles labeled with Cy5.5 fluorescent dye and containing antisense oligonucleotide as a payload. The magnetic iron oxide core enables tracking of the nanoparticles through in vivo magnetic resonance imaging, while Cy5.5 dye allows tracking by optical imaging. This report details the monitoring of the accumulation of this nanoparticle platform (termed MN-anti-miR10b) in orthotopically implanted glioblastoma tumors following intravenous injection. In addition, it provides insight into the in vivo delivery of RNA oligonucleotides, a problem that has hampered the translation of RNA therapeutics into the clinic.

Introduction

Glioblastoma multiforme (GBM) is the highest grade of astrocytoma for which there is virtually no cure. Approximately 15,000 people are diagnosed with glioblastoma annually, which has a dismal median survival of about 15 months and a 5-year survival rate of 5%¹. In the past decades, there has been marginal improvement in prognosis despite multiple efforts to advance therapeutic options. The current standard of care for GBM includes maximal surgical resection, when feasible, followed by radiotherapy and chemotherapy². Temozolomide (TMZ), the chemotherapy of choice, was the latest therapy for glioblastoma discovered to show notable clinical efficacy; however, at least 50% of GBM tumors show TMZ resistance³. In spite of this rigorous therapeutic regimen, there is still a significant clinical need for improved glioblastoma therapy.

The development of therapeutics for GBM and other brain-related diseases is significantly hampered by the selective nature of the blood-brain barrier (BBB). The BBB is a physiological barrier comprised of endothelial cells, pericytes, and astrocyte feet-ends, which creates the semi-permeable membrane between the circulatory system and the brain, restricting the free passage of molecules and cells into the brain⁴. While protective in normal physiology and critical for brain homeostasis, the BBB prevents many therapeutics from reaching the brain, complicating the treatment of GBM. Efforts to enhance the delivery of therapeutics to GBM have led to the development of nanoparticle-based delivery vehicles, focused ultrasound drug delivery enhancement, and receptor-mediated drug delivery^5,6.

Nanoparticles have emerged as a promising medium for developing therapeutics for a myriad of diseases, including cancers. The application of nanoparticles for imaging and therapeutic purposes in GBM has been attempted using various nanoparticle constructs^7,8. With the focus on delivering drugs to GBM in conjunction with in vivo imaging of the delivery, the proposed approach utilizes magnetic nanoparticles (MN) consisting of an iron oxide core and covered by dextran for stability. The magnetic properties of these nanoparticles afford for their detection by magnetic resonance (MR) imaging, while simple conjugation chemistry to the aminated dextran coating allows for conjugation of therapeutic moieties such as RNA molecules, additional targeting moieties, or imaging moieties (such as Cy5.5 near-infrared optical dye)^9,10. In addition to the imaging capabilities, the nano platform is able to extend the half-life of RNA therapeutics by protecting the oligonucleotide from endogenous nucleases, improving therapeutic delivery. Here, the application of this nano platform for in vivo delivery of therapeutic oligonucleotides (termed MN-anti-miR10b) to GBM, monitored by in vivo imaging, is presented. Previously, the ability of this nano platform to accumulate was demonstrated in GBM cells in vitro, causing significant loss of viability of tumor cells¹¹. Prior to performing therapeutic in vivo studies, it is necessary to demonstrate in vivo delivery of this nano platform to GBM tumors in animal models. To achieve this, orthotopic GBM animal models were produced, and intravenous administration of the construct was performed followed by in vivo imaging. Outlined here are the protocols of these studies showing accumulation in the tumor region confirmed by in vivo imaging and ex vivo microscopy.

Protocol

All procedures involving animal subjects have been approved by the Michigan State University Institutional Animal Care and Use Committee (IACUC). Female outbred athymic nude mice were purchased from Jackson Labs (strain #007850) at 7 weeks of age and allowed to acclimate for 1 week prior to implantation surgery. Mice were approximately 21-25 g at the time of implant. U251 cells expressing firefly luciferase were generated and provided by Dr. Ana deCarvalho¹².

1. Cell culture and preparation for implantation

1. Culture luciferase-labeled, human glioblastoma cells, U251 in Dulbecco's Modified Eagle Medium (DMEM) containing D-glucose, L-glutamine, and sodium pyruvate and supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin at 37 °C with 5% CO₂.
2. Once the cells reach 70% confluency, trypsinize with 0.25% trypsin/EDTA for 1-2 min at 37 °C. Use 2-4 mL for a T-75 flask, or 4-6 mL for a T-175 flask.
3. After cells have detached from the flask, quench the reaction with a 2:1 ratio of supplemented DMEM and mix gently by pipetting to dissociate cells into a single-cell suspension. Transfer the cells into a conical tube and pellet the cells by centrifugation at 300 x g for 5 min.
4. Carefully aspirate the supernatant and resuspend the cell pellet in 1x phosphate-buffered saline (PBS). Count the cells with a hemocytometer using trypan blue staining to exclude dead cells and calculate the total number of live cells. Pellet the cells again, as described above.
5. Resuspend the cell pellet to a concentration of 1 x 10⁸ cells/mL in PBS and store the cell suspension on ice for intracranial implantation.

2. Freehand orthotopic tumor implantation

NOTE: This protocol is adapted from Irtenkauf et al. (Dr. Ana deCarvalho's procedure; Henry Ford Health Hermelin Brain Tumor Center)¹². All steps should be carried out in a biosafety cabinet to ensure safety for both subjects and researchers. The freehand implantation method allows for a faster procedure to achieve larger sample sizes while maintaining the quality of intracranial implantation. Alternatively, a stereotaxic device can be used to implant the tumor at the same coordinates described below.

1. Weigh the nude, athymic mouse and administer an anesthetic solution of ketamine/xylazine (100 mg/kg/10mg/kg) by intraperitoneal injection with a 28G syringe. Place the mouse in a recovery cage on top of a heating pad to maintain body temperature. Confirm anesthetization by checking the pedal reflex.
2. Apply ophthalmic eye ointment, administer ketoprofen (5 mg/kg) subcutaneously with a 28G syringe, and give identifying ear punches.
3. Sterilize the surgical site at the top of the head with 70% ethanol followed by betadine scrub and repeat 3x. Then, make a 5-7 mm incision with small surgical scissors to the right of the midline of the head and retract the skin with fine forceps to expose the skull and suture lines of the skull.
4. Scrub the skull with a cotton swab to remove the periosteum, allowing access to the bone. Apply a solution of 3% hydrogen peroxide using a new cotton swab to dry out the bone. Using a new cotton swab, wipe away excess hydrogen peroxide and any foam that may form. Repeat this step 3x until the bregma is visible on the skull and the bone is sufficiently dry for drilling. A sufficiently dry skull will have starkly whitened suture lines and reduced redness from the starting appearance.
5. With respect to the bregma, measure -2.5 mm medial/lateral and -1 mm anterior/posterior with a ruler and mark the drill site with a marker. Measure again before drilling to ensure the mark is in the correct position.
6. Take an electric micro-surgery drill (11,000 RPM) with a 0.5 mm drill burr and carefully make a hole in the skull where previously marked. Absorb any blood or fluid that may accumulate at the drill site with a fresh cotton swab.
7. Prepare a 33G microliter syringe fitted with a needle sleeve to an exposed needle length of 3 mm. Draw up 5 µL of the previously prepared 1 x 10⁸ cell/mL cell suspension after briefly resuspending with a pipette and ensure there are no bubbles in the injection solution. Carefully wipe away any excess cell suspension left on the exterior of the needle and needle sleeve with a 70% isopropanol wipe.
8. Insert the needle vertically into the drill site until the needle sleeve stops further insertion of the needle. Carefully retract the needle approximately 0.5 mm to create space for the injected cells to accumulate.
9. Slowly inject the cell suspension over a period of 1 min. Once the full volume has been injected, hold the needle at depth for an additional minute to reduce the efflux of the injection solution. Then, retract the needle carefully.
10. With a clean cotton swab, wipe away any liquid that accumulates at the injection site and then apply bone wax to seal the hole.
11. Close the incision with a veterinary bond and allow it to dry. With a clean cotton swab, apply topical lidocaine as an analgesic.
12. Monitor the mouse until fully recovered and ambulatory. Administer ketoprofen (5 mg/kg) subcutaneously for 2 days post-implantation for pain management.

3. Nanoparticle platform synthesis

1. Synthesize and conjugate the nanoparticles with Cy5.5 and oligonucleotide (anti-miR10b) as previously described¹¹.

4. MN-anti-miR10b administration

NOTE: In this study, the injections were done once a week for 6 weeks beginning 7 days after implantation, but the same protocol can be used for various injection frequencies.

1. Weigh the mouse and calculate the volume of MN-anti-miR10b to administer to the mouse. Doses of MN-anti-miR10b are given at 20 mg Fe/kg in murine GBM models. For example, a 20 g mouse receives an 80 µL dosage of 5 mg Fe/mL MN-anti-miR10b. Control mice receive an equal volume of PBS consistent with this dosage scheme.
2. Prepare a 28G syringe with the dose of MN-anti-miR10b and ensure there are no bubbles in the injection solution.
3. Anesthetize the mouse under 2%-4% isoflurane for induction and 1.5%-2% for maintenance with a 1.5 L/min flow rate in an induction box. Move the mouse to a nose cone and continue to administer anesthesia. Check the depth of anesthesia and apply vet ointment.
4. Sterilize the tail with a 70% isopropanol wipe and allow excess alcohol to dry.
5. Rotate the tail to position the lateral caudal veins to the top of the tail. Then, aiming for the vein, insert the needle into the tail and establish a vacuum by pulling back the plunger.
6. Insert the needle until blood enters the syringe, indicating successful entry into the vein.
7. Slowly inject MN-anti-miR10b and retract the needle after complete delivery. Apply pressure to the injection site with gauze until the bleeding stops.
8. Remove the mouse from the nose cone. Monitor the mouse until fully recovered and ambulatory.
   NOTE: Bolus administration of nanoparticles may cause respiratory distress. Carefully monitor the mouse and immediately apply light chest compressions if there are signs of respiratory distress.

5. In vivo bioluminescence and fluorescence imaging

NOTE: In vivo bioluminescence and fluorescence imaging are conducted in the In Vivo Imaging System (IVIS) before and 24 h after the nanoparticle is injected.

1. Prepare sterile-filtered D-luciferin at a concentration of 30 mg/mL in PBS without Mg²⁺ and Ca²⁺.
2. Open the IVIS operation software and begin initialization to prepare the machine. While the system initializes, select a folder to save images by selecting Acquisition > Auto-Save To and define the folder for the files to be saved.
3. At imaging time points, weigh the mouse and calculate the volume of D-luciferin to administer for a dosage of 150 mg/kg. Prepare a 28G syringe with the volume, protecting the solution from direct light. This study included imaging 2 days after implantation surgery to confirm seeding, and weekly imaging started on day 7 post-implant immediately prior to weekly treatments.
4. Anesthetize the mouse under 2%-4% isoflurane for induction and 1.5%-2% for maintenance with a 1.5 L/min flow rate in an induction chamber. Once anesthetized, gently scruff the mouse to avoid further injuring the surgical site and inject the dose of D-luciferin intraperitoneally. Allow the mouse to recover and wait for 10 min before imaging to allow the bioluminescence signal to develop and stabilize.
5. While the bioluminescence signal develops, prepare the IVIS scanner for bioluminescence imaging. Place down the black low fluorescence mat on the imaging stage and configure the nose cone array for the number of subjects to be imaged.
6. In the software, click Imaging Wizard. Select Bioluminescence Imaging and then select Open Filter. On the next screen, select Imaging Subject and Field of View. The IVIS is now set for bioluminescence imaging.
7. At 3 min before imaging, begin priming the induction box by allowing the isoflurane/oxygen mixture to fill the box. At 2 min before imaging, place mice in the induction box for anesthetization.
8. Once anesthetized, move the mice from the induction box to the IVIS for imaging and lay each down in the prone position. Provide maintenance anesthesia using 2% isoflurane during imaging.
9. Once mice have been placed in the IVIS scanner, click Acquire Sequence. Take an image using auto-exposure settings with a minimum signal threshold of 3,000 counts. Bioluminescence imaging visualizes the localization of luciferase-labeled U251 glioblastoma cells. Auto-exposure will capture an image with an exposure length calculated based on the brightest signal in view up to the user-defined maximum length of 5 min.
   NOTE: A typical cage of mice requires a single image, as seeding is generally uniform. In cases with one or more bright mice, mice may be removed, and a repeat acquisition can be taken to better capture signals from dim mice.
10. After bioluminescence imaging, change the IVIS settings to prepare epi-fluorescence imaging and take fluorescence scans.
    1. In the Imaging Wizard, select Fluorescence > Filtered Pair with Epi-Illumination. In the next screen, select the probes to be imaged, such as Cy5.5. Select the Imaging Subject and Field of View. The IVIS scanner is now set for fluorescence imaging of the probes selected in the previous screen.
11. Take an image using auto-exposure settings with a minimum signal threshold of 6,000 counts. Cy5.5 fluorescence imaging visualizes the localization of MN-anti-miR10b. Auto-exposure will capture an image with an exposure length calculated based on the brightest signal in view up to the user-defined maximum length of 5 min.
    NOTE: Fluorescence imaging rarely takes longer than 1 min exposures, even in control PBS-injected mice. A single image is always sufficient as the signal deviation between treated mice is small.
12. Remove the mouse from the IVIS after imaging. Monitor until fully recovered from anesthesia and ambulatory.

6. In vivo magnetic resonance imaging

NOTE: MR imaging is performed before and 24 h after nanoparticle injection and can be performed in the same animals that undergoes optical and bioluminescence imaging.

1. Anesthetize the subject mouse in an induction box under 2%-4% isoflurane for induction and 1.5%-2% for maintenance with a 1.5 L/min flow rate. Once anesthetized, place the mouse prone on the MRI bed on top of the respiratory monitoring balloon. Fit the nose cone snuggly to the snout to deliver anesthesia during imaging and apply ophthalmic eye ointment.
2. Immobilize and position the head for scanning using a bite bar and ear bars.
3. Install the lubricated rectal temperature probe and ensure respiration and temperature monitoring are functioning.Position the mouse brain coil over the head by fitting the pegs on the mouse bed into the holes on the coil and tape the coil into place to reduce movement during scanning.
4. Place a small warm water circulating pad over the top of the mouse to maintain body temperature while anesthetized. Take care to allow some distance between the coil and the heating blanket to limit distortion of the image by water interference.
5. Move the mouse and imaging bed into position for scanning.
6. Tune and match the MRI coils by starting the Wobble setup step in the acquisition software. At the service end of the MRI, use the tune and match knobs on the coil to ensure that the trace is centered (tune) and as deep as possible (match).
7. Acquire a three-plane localizer scan and adjust the imaging bed to position the brain at the isocenter of the magnet, if necessary.
8. Acquire 2D T₂ weighted scans to detect the injected agent in the tumor with the following parameters: repetition time (TR) = 2500 ms, echo time (TE) = 25 ms, the field of view (FOV) 20 x 20 mm, 18 coronal slices, 0.2 mm slice gap, 150 x 150 µm in-plane resolution, 0.5 mm slice thickness.
9. Acquire a B0 map of the whole brain to calculate a localized shim using the Mapshim utility. Then, use 3D T₂ weighted images to visualize the nanoparticles with TR = 30 ms, TE = 10 ms, FOV 20 x 15 x 12 mm, resolution 100 µm isotropic.
10. Acquire T₂^* map for further nanoparticle imaging using the 2D T₂ weighted image as a reference to position scan over the tumor. Use TR = 800 ms, TE = 3.5 ms. FOV 20 x 20 mm, 5 coronal slices, no slice gap. Resolution in plane 100 µm in plane. Acquire 10 positive echo images with 5 ms echo time spacing.
11. Monitor respiration and body temperature throughout the imaging sequences and adjust isoflurane and water temperature if necessary.
12. After imaging, remove the mouse from the MRI scanner and place it in a heated recovery cage. Monitor the mouse until it is fully recovered from anesthesia and ambulatory.

7. Ex vivo bioluminescence and fluorescence imaging

1. Perform ex vivo bioluminescence and fluorescence imaging in the IVIS, similar to the in vivo imaging. Carry out the IVIS initialization and scan setting steps as described above.
2. At the experimental endpoint, weigh the mouse and calculate the volume of D-luciferin to administer for a dosage of 150 mg/kg. Inject the dose of D-luciferin and conduct live imaging 10 min post-injection, as previously described.
3. After final in vivo bioluminescence and fluorescence imaging, quickly euthanize the mouse by cervical dislocation while still under anesthesia, dissect the mouse, and collect the major organs, including the brain, on a Petri dish.
4. Place the Petri dish with excised organs in the IVIS scanner and image the organs with both bioluminescence and fluorescence modalities using the same acquisition settings as the in vivo imaging.
5. Flash freeze any organs necessary for further analysis in OCT by slowly lowering the cryomold with the specimen in OCT into a shallow pool of liquid nitrogen with long forceps until the OCT turns completely white. Do not allow liquid nitrogen to touch liquid OCT or lower the mold too quickly, as bubbling of the OCT will occur. Store frozen samples at -80 °C.
6. Store the brain containing the tumor in OCT and flash freeze for cryosectioning. Store at -80 °C until needed for analysis.

8. Fluorescence microscopy

1. Prepare the cryostat for cryosectioning. Set the chamber and specimen head temperatures between -15 °C and -20 °C.
2. Take the flash-frozen brain in OCT out of -80 °C storage and place it inside the cryostat chamber. Allow the samples to warm to the chamber temperature (-20 °C) before sectioning.
3. Using a single edged razor cut the brain coronally at the injection site. This is usually visible as a dimple on the surface of the brain. Mount the sample onto the specimen disc using a small amount of OCT to position the cut side of the brain to be sectioned. Apply ample pressure with the chilled weight inside the chamber to ensure stable mounting of the sample.
4. Move the mounted sample and specimen disc onto the specimen head in preparation for sectioning. Trim the tissue to the desired depth by taking 20 µm sections, adjusting the angle of the sample to section the tissue as near to level with the tissue as possible.
5. Once the desired tissue depth has been reached, adjust the cryostat to take 5 - 7 µm sections of the sample. Mount the sections onto glass slides pre-labeled with descriptors such as mouse ID, tissue sectioned, and slide number by quickly lowering the charged side of the microscope slide onto the section.
6. Make fresh 4% paraformaldehyde (PFA). Submerge the slide with tissue in the PFA solution for 15 min to fix the tissue. Rinse the slide with DPBS 3x after fixation.
7. To the now fixed tissue section on the glass slide, add a 40 µL drop of mounting medium containing 4',6-diamidino-2-phenylindole (DAPI), which stains cell nuclei. Carefully place a 24 mm x 50 mm glass coverslip on top of the mounting media and ensure that the entire tissue has been encased in the mounting media.
8. Visualize the tissue using DAPI (emission (em) at 359 nm, excitation (ex) at 457 nm) and Cy5.5 (ex 683 nm, em 703 nm) fluorescence microscopy.

Results

MN-anti-miR10b was synthesized and characterized, as described previously¹¹. Transmission electron microscopy of MN-anti-miR10b shows the morphology and polydispersity of the nano platform (Figure 1B). This nano platform has an average size of 25.12 ± 0.34 nm with a zeta potential of 13.18 ± 1.47 mV (Figure 1C,D). In these studies, nude athymic mice were orthotopically implanted with U251 human ...

Discussion

Several critical steps across the different methods of validating the accumulation of the nanoparticles across the BBB can be decisive for the success of the protocol. Beginning with the orthotopic implantation of GBM cells, it is important to ensure that the suture lines of the skull are visible after drying the bone; this aids in the accurate placement of the tumor cells. For drilling through the skull, it is best to apply light pressure to the drill site and begin drilling to make a shallow impression in the bone. Onc...

Materials

Name	Company	Catalog Number	Comments
Athymic nude "J:NU" mice	Jackson Laboratory	RRID:IMSR_JAX:007850	Immunocompromised mouse model
0.25% Trypsin	Gibco	25200-056	Cell culture reagent for U251
1.7 mL microcentrifuge tube	DOT Scientific	RN1700-GMT	For tissue collection
10 µL, Neuros Syringe, Model 1701 RN, 33 gauge, Point Style 4	Hamilton	65460-06	Syringe for intracranial implantation of tumor cells
3M Vetbond	3M	1469SB	Tissue adhesive for surgical site closure
4% Paraformaldehyde	Thermo Scientific	J199943-K2	Tissue fixing solution
70% isopropoyl alcohol wipe	Cardinal	MW-APL	Topical antiseptic wipe for tumor implantation and tail vein injection
Aperio Versa	Leica		For scanning of stained tissue section slides
Betadine Surgical Scrub	Purdue	6761815101	Topical antiseptic for tumor implantation
BioSpec 70/30	Bruker		Magnetic resonance imaging scanner
Bone Wax	Medline	DYNJBW25	Bone wax for sealing implantation site
Burrs for Micro drill	F.S.T.	19007-05	Drill burr used to make hole in skull for tumor implantation
DAPI Fluoromount-G	SouthernBiotech	0100-20	Tissue mounting media containing DAPI stain
Dulbecco’s Modified Eagle Medium (DMEM)	Gibco	11995-065	Cell culture media for U251
Extra Fine Graefe Forceps	F.S.T.	11150-10	Sugical tool for tumor implantation
Fetal bovine serum	Corning	35-010-CV	Cell culture media supplement for U251
Fine Scissors - Sharp 10.5cm	F.S.T.	14060-10	Sugical tool for tumor implantation
Glydo (Lidocaine)	Sagent	673-76	Topical analgesic for surgical site
Ideal Micro Drill	CellPoint Scientific	67-1200A	Drill used to make hole in skull for tumor implantation
Insulin syringe 1CC 29G X 1/2"	Becton, Dickinson	324704	Syringe for D-Luciferin injection and tail vein injection of nanoparticles
Isoflurane	Covetrus	11695067772	Anethesia
Isoflurane vaporizer	SOMNI Scientific	VS6002	Anethesia apparatus
IVIS SpectrumCT In Vivo Imaging System	PerkinElmer/Revvity	128201	Bioluminescence and fluorescence imaging scanner
IVISbrite D-Luciferin Potassium Salt	PerkinElmer/Revvity	122799-100MG	Substrate for bioluminescence imaging
Ketaset (Ketamine)	Zoetis	10004027	Anesthetic for tumor implantation surgery
Ketofen (Ketoprofen)	Zoetis	10004031	Analgesic for tumor implantation surgery
Leica CM1950	Leica	CM1950	For cryosectioning of OCT-embedded samples
PBS	Gibco	14190-144	Cell culture reagent and cell suspension solution for implantation of U251
Penicillin-streptomycin	Gibco	15140-122	Antibiotic for cell culture media for U251
Puralube vet ointment	MWI Veterinary	27505	Opthalmic eye ointment for protection during tumor implantation
Ruler	F.S.T.	18000-30	Used to measure drill site for implanation
Tissue-Tek Cryomold  Intermediate 15 x 15 x 5 mm	Sakura	4566	Collection mold for collecting tissue samples
Tissue-Tek O.C.T. Compound	Sakura	4583	Freezing compound for collecting tissue samples
U-251 MG cell line human	Millapore Sigma	9063001	Human glioblastoma cell line
Xylazine Injectable Solution, 100 mg/ml	Covetrus	1XYL006	Paralytic for tumor implantation surgery