training-laboratory-rabbits-to-refine-routine-husbandry-procedures

Training Laboratory Rabbits to Refine Routine Husbandry Procedures

Non-aversive handling and training techniques for laboratory animals are required to facilitate experimental and routine husbandry procedures, improving ...

German Federal Institute for Risk Assessment

Accurate detection of the onset of parturition is a key factor in the prevention of dystocia. In order to establish current best practice recommendations for calving management, we asked subject matter experts (SME) who had published on calving management (by online survey,  = 80) and non-SMEs, veterinary practitioners (by workshop survey,  = 24) for their opinions. For this, we designed a questionnaire on the significance of signs of imminent parturition (SIP), the frequency of calving observation, and influencing factors for the timing of cow movement to a maternity pen. The response rate was 67.5% in the online survey and 100% in the workshop survey. The majority (89.7%) of all respondents agreed that it is beneficial for successful calving management to differentiate between stage I and II of parturition. Of 12 signs of imminent parturition (for stage I and II), "restlessness" and "visibility of fetal parts in vulva" were cited by 56.5% and 73.3% of SME and non-SME respondents, respectively. There was no consensus on the right time to move the cow to the maternity pen; recommendations varied from one to over 21 days. Almost half of the respondents (45.7%) recommended a 6-h observation interval for prepartum cows in the maternity pen. This study identified a strong consensus on the SIP and how and when to observe cows prior to parturition. SMEs and non-SMEs provided broadly similar recommendations, while the SMEs and the non-SMEs differed significantly in the number of publications on calving they authored, they differed little in their knowledge of calving management.

Calving Management: A Questionnaire Survey of Veterinary Subject Matter Experts and Non-Experts.

Boredom is an emotional state that occurs when an individual has nothing to do, is not interested in the surrounding, and feels dreary and in a monotony. While this condition is usually defined for humans, it may very well describe the lives of many laboratory animals housed in small, barren cages. To make the cages less monotonous, environmental enrichment is often proposed. Although housing in a stimulating environment is still used predominantly as a luxury good and for treatment in preclinical research, enrichment is increasingly recognized to improve animal welfare. To gain insight into how stimulating environments influence the welfare of laboratory rodents, we conducted a systematic review of studies that analyzed the effect of enriched environment on behavioral parameters of animal well-being. Remarkably, a considerable number of these parameters can be associated with symptoms of boredom. Our findings show that a stimulating living environment is essential for the development of natural behavior and animal welfare of laboratory rats and mice alike, regardless of age and sex. Conversely, confinement and under-stimulation has potentially detrimental effects on the mental and physical health of laboratory rodents. We show that boredom in experimental animals is measurable and does not have to be accepted as inevitable.

Bored at home?-A systematic review on the effect of environmental enrichment on the welfare of laboratory rats and mice.

Lupin varieties with a low content of quinolizidine alkaloids (QAs) like blue sweet lupin (BSL) have long been used as a protein source for dairy cows. A health concern for humans may arise from the transfer of acute toxic QAs from feed into cow's milk. This study is the first to quantify the transfer of QAs from BSL into cow's milk with experimental and modeling methods. Four lactating dairy cows were subjected to two 7 day feeding periods with 1 and 2 kg/d BSL, respectively, each followed by a depuration period. BSL contained 1774 mg/kg dry matter total QAs. Individual milk samples were taken twice daily and QA contents in feed and milk determined with liquid chromatography-tandem mass spectrometry. Transfer of QAs into the milk was already seen with the administration of 1 kg/d BSL, with differences in transfer rates (TRs) between individual QAs. A toxicokinetic model was derived to quantify and predict QA feed-to-food transfer. For the four most prominent QAs, our model shows an α-half-life of around 0.27 d. TRs were obtained for six QAs and were between 0.13 (sparteine) and 3.74% (multiflorine). A toxicological assessment of milk containing QAs as measured in this study indicated a potential health concern.

Investigations on the Transfer of Quinolizidine Alkaloids from  into the Milk of Dairy Cows.

Facial expression scoring has proven to be useful for pain evaluation in humans. In the last decade, equivalent scales have been developed for various animal species, including large domestic animals. The research question of this systematic review was as follows: is facial expression scoring (intervention) a valid method to evaluate pain (the outcome) in large domestic animals (population)?

"Do not look at me like that": Is the facial expression score reliable and accurate to evaluate pain in large domestic animals? A systematic review.

Campylobacteriosis outbreaks have repeatedly been associated with the consumption of raw milk. This study aimed to explore the variation in the prevalence and concentration of  spp. in cows' milk and feces, the farm environment and on the teat skin over an entire year at a small German dairy farm. Bi-weekly samples were collected from the environment (boot socks), teats, raw milk, milk filters, milking clusters and feces collected from the recta of dairy cows. Samples were analyzed for  spp., , the total aerobic plate count and for  spp. The prevalence of  spp. was found to be the highest in feces (77.1%), completely absent in milking equipment and low in raw milk (0.4%). The mean concentration of  spp. was 2.43 log colony-forming units (CFU)/g in feces and 1.26 log CFU/teat swab. Only a single milk filter at the end of the milk pipeline and one individual cow's raw milk sample were positive on the same day, with a concentration of 2.74 log CFU/filter and 2.37 log CFU/mL for the raw milk. On the same day, nine teat swab samples tested positive for  spp. This study highlights the persistence of  spp. for at least one year in the intestine of individual cows and within the general farm environment and demonstrates that fecal cross-contamination of the teats can occur even when the contamination of raw milk is a rare event.

Longitudinal Study for the Detection and Quantification of  spp. in Dairy Cows during Milking and in the Dairy Farm Environment.

Hypoglycin A (HGA), methylenecyclopropylglycine (MCPrG), hypoglycin B (HGB), and γ-glutamyl-α-(methylenecyclopropyl) glycine (γ-glutamyl-MCPrG) are secondary plant metabolites occurring in sycamore maple () as well as several other Sapindaceae (e.g., ). By interfering with energy metabolism, they may cause severe intoxication in humans and other species. However, to date, there is not enough data available concerning the intake, metabolism, or excretion of sycamore maple toxins in dairy cows. In May 2022, five cows were observed over four days, when they had first access to a pasture with two sycamore maples. Grazing of their seedlings that grew numerously in between the pasture plants was monitored by direct observation. Milk samples were drawn both from individual cows and from the bulk tank. Spontaneous urine samples were collected from all cows on day 3 after access to the pasture. Seedlings (100 g) were sampled on the pasture and analyzed, together with milk and urine samples, for sycamore toxins and their metabolites using liquid chromatography-tandem mass spectrometry and liquid chromatography-high-resolution mass spectrometry. Cows ingested sycamore seedlings while grazing. Values of HGA in milk were below the limit of quantification. However, metabolites of HGA and MCPrG were detected in individual milk samples already at the end of the first day of grazing. Urine samples of all five cows showed higher concentrations of conjugated HGA and MCPrG metabolites than in milk. Observations suggest that dairy cows may have a low susceptibility toward sycamore maple toxins. However, whether this could be attributed to foregut fermenting species in general requires further elucidation.

Detection of Hypoglycin A and MCPrG Metabolites in the Milk and Urine of Pasture Dairy Cows after Intake of Sycamore Seedlings.

Dry cow treatment with an intramammary antibiotic is recommended to reduce the risk of mastitis at the beginning of the next lactation. The dry period may be shortened unintentionally, affecting antibiotic residue depletion and the time when residues reach concentrations below the maximum residue limit (MRL). The objective of this study was to evaluate residue depletion in milk after dry cow treatment with cloxacillin, considering dry periods of 14 (G14d), 21 (G21d), and 28 d (G28d). Overall, fifteen cows with 60 udder quarters were included in the study. For each cow, three of the udder quarters were treated with 1000 mg cloxacillin benzathine (2:1) on d 252, d 259, and d 266 of gestation; one quarter was left untreated. Milk samples were drawn until 20 DIM and milk composition, somatic cell count and cloxacillin residues were analyzed. The HPLC-MS/MS revealed different excretion kinetics for the compounds cloxacillin and cloxacillin benzathine (1:1). All cows showed a cloxacillin and cloxacillin benzathine (1:1) concentration below the MRL of 30 µg/kg after 5 d. In the udder quarters of G21d and G28d, the cloxacillin concentration was already below the MRL at first milking after calving. The cloxacillin benzathine (1:1) concentration in the milk of G28d, G21d, and G14d fell below 30 µg/kg on the 5th, 3rd, and 5th DIM, respectively. Shortening the dry period affects residue depletion after dry cow treatment with cloxacillin. The risk of exceeding the MRL, however, seems low, even with dry periods shorter than 14 d.

Residue Concentrations of Cloxacillin in Milk after Intramammary Dry Cow Treatment Considering Dry Period Length.

Consensus on optimal calving management is difficult to achieve among veterinarians. Some of this discordance may stem from asymmetry between the literature written by subject matter experts (SME) and the knowledge, attitude and practice (KAP) of non-SMEs and veterinary practitioners. Hence, the objective of this study was to assess how veterinary practitioners KAPs in calving management compared with the findings in the literature. An online questionnaire survey was conducted prior to a World Buiatrics Congress by delegates (n = 80) who attended a workshop on calving management at which the outcomes of the latter were recorded. The responses from the questionnaire and the consensus from the workshop were compared and these were compared with the literature on calving management, specifically, prediction of calving, assistance at calving and farmer training in calving management. In general, there was good agreement between the aggregated individual responses to the questionnaire and the consensuses from the workshop. The suggested methods of predicting calving were well aligned between practitioners and those researched in the literature but practitioners tended to emphasize more practical aspects of prediction while the literature tends to focus more on the effectiveness of various predictor devices. Practitioners tended to recommend a later time of calving assistance than that recommended in the recent literature though they did also emphasize the importance of calving progress. The majority of practitioners conducted informal (at calving) calving training focusing on time of intervention, welfare-friendly intervention and delivery of a live healthy calf. However, the educational literature recommends a formal training approach as being more effective. These findings indicate that practitioners may benefit from continuing professional education on the scientific merits/demerits of calving prediction devices, current best practice on intervention timing during calving and formal methods of farmer training on calving management.

Carola Fischer-Tenhagen

German Centre for the Protection of Laboratory Animals (Bf3R)

German Federal Institute for Risk Assessment

Training Laboratory Rabbits to Refine Routine Husbandry Procedures