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An Unbiased Approach of Sampling TEM Sections in Neuroscience

7.1K Views

10:56 min

April 13th, 2019

DOI :

10.3791/58745-v

April 13th, 2019


Transcript

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Unbiased Sampling Electron Microscopy Neuroscience Quantification Numerical Density Synapses Neural Network Sample Preparation Ultramicrotome Serial Sections Slot Grid Counterstaining Transmission Electron Microscope TEM TEM Serial Section Software Region Of Interest ROI Random Point Sampling

Chapters in this video

0:04

Title

1:02

TEM Preparation Embedding, Ultra-Thin Sectioning and Staining

2:05

Imaging of Corresponding ROIs on the Reference and Look-Up Sections on TEM with Software Packages

5:25

Analyze the TEM images with ImageJ to Document Ultrastructural Features

7:10

Using SerialEM Script to Optimize Elemental Analysis in Brain Samples in Combination with DigitalMicrograph

9:19

Results: Unbiased Approach of Sampling TEM Sections

10:25

Conclusion

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