JOVE JOURNAL

生物化学

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Metal Ion Addition and Solvent Exchange Approaches to ECF-5 Peptide Hydrogel Formation for Enhanced Biomedical Applications

Journal (Biochemistry)

Metal Ion Addition and Solvent Exchange Approaches to ECF-5 Peptide Hydrogel Formation for Enhanced Biomedical Applications

Yue Zhao¹, Mai Hu², Yiwen Gong², Yihao Zhao², Zhiwei Shen²

¹The First Affiliated Hospital of Nanchang University, ²The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University

Preparation of ECF-5 Peptide Hydrogels for Regenerative Medicine Using pH Response Method

Journal (Biochemistry)

Preparation of ECF-5 Peptide Hydrogels for Regenerative Medicine Using pH Response Method

Yue Zhao¹, Mai Hu², Yiwen Gong², Yihao Zhao², Zhiwei Shen²

¹The First Affiliated Hospital of Nanchang University, ²The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University

Atomic Force Microscopy to Characterize the Fiber Morphology of ECF-5 Peptide Hydrogels for Tissue Engineering

Journal (Biochemistry)

Atomic Force Microscopy to Characterize the Fiber Morphology of ECF-5 Peptide Hydrogels for Tissue Engineering

Yue Zhao¹, Mai Hu², Yiwen Gong², Yihao Zhao², Zhiwei Shen²

¹The First Affiliated Hospital of Nanchang University, ²The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University

Nucleosome Reconstitution for Single-Molecule Chromatin Studies

Journal (Biochemistry)

Nucleosome Reconstitution for Single-Molecule Chromatin Studies

Htet Ng*¹, Masuda Begum*¹, Gabriella N. L. Chua*¹^,², Shixin Liu¹

¹Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, ²Tri-Institutional PhD Program in Chemical Biology

Preparing Peanut Worm Homogenate and Gel for Fibrinogen-PAGE to Identify Fibrinogenolytic Agents

Journal (Biochemistry)

Preparing Peanut Worm Homogenate and Gel for Fibrinogen-PAGE to Identify Fibrinogenolytic Agents

Bin Kang*¹, Chengjia Hu*², Hongjun Lin², Hui Yan², Chengyun Wei³, Mingqing Tang²

¹Department of Laboratory Medicine, Quanzhou First Hospital Affiliated to Fujian Medical University, ²Medicine School, Huaqiao University, ³Department of Laboratory Medicine, Hospital of Huaqiao University

Fibrinogen-PAGE for Fibrinogenolytic Agent Analysis in Peanut Worm Homogenate

Journal (Biochemistry)

Fibrinogen-PAGE for Fibrinogenolytic Agent Analysis in Peanut Worm Homogenate

Bin Kang*¹, Chengjia Hu*², Hongjun Lin², Hui Yan², Chengyun Wei³, Mingqing Tang²

¹Department of Laboratory Medicine, Quanzhou First Hospital Affiliated to Fujian Medical University, ²Medicine School, Huaqiao University, ³Department of Laboratory Medicine, Hospital of Huaqiao University

Preparation of Human Telomeric Restriction Fragments and Measurement of Telomeric DNA Using Single-Molecule Magnetic Tweezers

Journal (Biochemistry)

Preparation of Human Telomeric Restriction Fragments and Measurement of Telomeric DNA Using Single-Molecule Magnetic Tweezers

Han Gao¹, Yanling Liu¹, Zhongbo Yu¹

¹State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University

Expression and Purification of Telomeric DNA-Binding Protein TRF2 Using SUMO Fusion Tag

Journal (Biochemistry)

Expression and Purification of Telomeric DNA-Binding Protein TRF2 Using SUMO Fusion Tag

Han Gao¹, Yanling Liu¹, Zhongbo Yu¹

¹State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University

Ensemble Assembly and Activation of Fluorescently Labeled CMG onto Magnetic Bead-Bound DNA for Single Molecule Imaging

Journal (Biochemistry)

Ensemble Assembly and Activation of Fluorescently Labeled CMG onto Magnetic Bead-Bound DNA for Single Molecule Imaging

Daniel Ramírez Montero¹, Humberto Sánchez¹, Edo van Veen¹, Theo van Laar¹, Belén Solano¹, John F. X. Diffley², Nynke H. Dekker¹^,³

¹Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, ²Chromosome Replication Laboratory, Francis Crick Institute, ³Department of Physics and Kavli Institute of Nanoscience Discovery, University of Oxford

Single-Molecule Imaging of Fully Reconstituted CMG with Correlative Dual-Beam Optical Tweezers and Confocal Microscopy

Journal (Biochemistry)

Single-Molecule Imaging of Fully Reconstituted CMG with Correlative Dual-Beam Optical Tweezers and Confocal Microscopy

Daniel Ramírez Montero¹, Humberto Sánchez¹, Edo van Veen¹, Theo van Laar¹, Belén Solano¹, John F. X. Diffley², Nynke H. Dekker¹^,³

¹Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, ²Chromosome Replication Laboratory, Francis Crick Institute, ³Department of Physics and Kavli Institute of Nanoscience Discovery, University of Oxford

Binding of Doubly Functionalized Linear DNA Substrate to Streptavidin-Coated Magnetic Beads

Journal (Biochemistry)

Binding of Doubly Functionalized Linear DNA Substrate to Streptavidin-Coated Magnetic Beads

Daniel Ramírez Montero¹, Humberto Sánchez¹, Edo van Veen¹, Theo van Laar¹, Belén Solano¹, John F. X. Diffley², Nynke H. Dekker¹^,³

¹Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, ²Chromosome Replication Laboratory, Francis Crick Institute, ³Department of Physics and Kavli Institute of Nanoscience Discovery, University of Oxford

Chemical Treatment and Luciferase Assay to Measure the Level of ER-Mitochondria Contacts in Live Cells

Journal (Biochemistry)

Chemical Treatment and Luciferase Assay to Measure the Level of ER-Mitochondria Contacts in Live Cells

Connie Chen*¹, Katherine A. Rafael*¹, Ginam Cho¹, Youngshin Lim¹^,²

¹Department of Pathology, Cedars-Sinai Medical Center, ²Department of Biomedical Science Education, College of Medicine, Charles R. Drew University of Medicine and Science

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