Begin by installing the solvent-assisted flavor evaporation, or SAFE, glass assembly. Then, connect the distillation bottle at the lower left connection site and the collection bottle at the lower right connection site of the assembly. Connect the circulating water tube to the rear portion of the assembly, ensuring the inlet enters the top and the outlet exits from the bottom.
After installing the cold trap, connect the upper right opening of the trap to the vacuum pump with a tube. Set the temperature of the circulating water to 50 degrees Celsius and that of the water bath for the sample distillation flask to 40 degrees Celsius. Then, close the vacuum valve.
Switch on the vacuum pump to initiate the vacuum application. To prepare for sample distillation, first, start the water circulation. Add liquid nitrogen into the cold trap, as well as surround the collecting bottle with liquid nitrogen.
Pour the tea infusion into the sample funnel at the top left and then cover it with a glass stopper. Next, gradually introduce the sample into the distillation flask drop-wise. Control the sample drop speed to maintain the vacuum within the proper range.
After the distillation completes, press the control button of the vacuum pump. And when stop flashes, press the Enter key to confirm. When the speed of the vacuum pump decreases to zero, unplug the power cord.
Remove the grinding plug above the sampling bottle. Then, unscrew the knob of the vacuum valve slowly to restore the system to atmospheric pressure. Once atmospheric pressure is restored in the system, remove the liquid nitrogen surrounding the collection bottle.
Slowly, unscrew the collection bottle and carefully remove the bottle containing the sample. Finally, stop the water circulation.
