Begin by placing the anesthetized animal on the operation table. Disinfect the skin with three alternate swabs of Iodophor disinfecting solution and 75%alcohol. Use two hemostats to lift the skin in the middle of the abdomen.
Use a scalpel to cut through the abdominal skin and muscles from the raphe to the pubic synthesis. After opening the abdominal cavity, separate the bowel with sterile cotton balls. To expose the abdominal aorta in the posterior abdominal wall, locate the abdominal aorta and take five milliliters of rat blood into blood collection tubes.
Keep the tubes containing the blood standing for one hour and centrifuge at 3000 G for 10 minutes to obtain the plasma. To collect the gastrocnemius muscle, cut open the skin with surgical scissors along the lower abdominal opening toward the lateral aspect of the right lower limb, and expose the lower limb muscles. After carefully separating the fascia with forceps, cut using a scalpel to remove the intact gastrocnemius muscle.
Wash the gastrocnemius muscle in sterile saline to remove adhering hair and blood, and place the removed gastrocnemius muscle into a 15 milliliter centrifuge tube containing 4%Paraformaldehyde.
