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Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds

11.6K Views

18:25 min

August 25th, 2013

DOI :

10.3791/50531-v

August 25th, 2013


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Live Imaging

Kapitel in diesem Video

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Title

1:41

Transcriptional Silencing of ASM

4:18

Preparation of Reagents for Live Microscopy

5:29

Live Cell Imaging of FM1-43 Influx into SLO Treated and Untreated Cells

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Quantification of FM1-43 Influx into Cells

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Results: Ca2+ and Sphingomyelinase are Involved in Plasma Membrane Repair

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Conclusion

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