College of Charleston

2 ARTICLES PUBLISHED IN JoVE

Genetics

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

Sophia Emetu^*1, Morgan Troiano^*1, Jacob Goldmintz^*1, Jensen Tomberlin¹, Simon Grelet², Philip H. Howe², Christopher Korey³, Renaud Geslain¹

¹Laboratory of tRNA Biology, Department of Biology, College of Charleston, ²Department of Biochemistry and Molecular Biology, MUSC, ³Department of Biology, College of Charleston

We describe an original transfer RNA analysis platform named SPOt (Streamlined Platform for Observing tRNA). SPOt simultaneously measures cellular levels of all tRNAs in biological samples, in only three steps, and in less than 24 hours.

Education

Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry

Tuguldur T. Odbadrakh¹, Ariel G. Gale¹, Benjamin T. Ball¹, Berhane Temelso², George C. Shields¹

¹Department of Chemistry, Furman University, ²College of Charleston

The atmospheric concentrations of weakly bound molecular clusters can be computed from the thermochemical properties of low energy structures found through a multi-step configurational sampling methodology utilizing a genetic algorithm and semi-empirical and ab initio quantum chemistry.

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