S'identifier

C3M, Université Côte d'Azur, Inserm

3 ARTICLES PUBLISHED IN JoVE

Biology

Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging

Jérôme Gilleron¹, Cindy Meziat¹, André Sulen², Stoyan Ivanov³, Jennifer Jager¹, David Estève⁴, Catherine Muller⁴, Jean-Francois Tanti¹, Mireille Cormont¹

¹Université Côte d'Azur, Inserm UMR1065, C3M, Team "Cellular and Molecular Pathophysiology of Obesity", Nice, France, ²Integrated Cardio Metabolic Center, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, ³Université Côte d'Azur, Inserm UMR1065, C3M, Team "Haematometabolism in Diseases", Nice, France, ⁴Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France

Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.

Biology

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Jennifer Jager¹, Mélanie Gaudfrin¹, Jérôme Gilleron¹, Mireille Cormont¹, Jean-François Tanti¹

¹Université Côte d’Azur, Inserm, C3M, Team Cellular and Molecular Pathophysiology of Obesity and Diabetes, Nice, France

Presented here is a protocol to deliver oligonucleotides such as small-interfering RNA (siRNA), micro-RNA mimics (miRs), or anti-micro-RNA (anti-miR) into mature adipocytes to modulate protein and micro-RNA expression.

Exploring Adipose Tissue Functions

Jennifer Jager¹, Jérôme Gilleron¹

¹Team Cellular and Molecular Pathophysiology of Obesity and Diabetes, C3M, Université Côte d'Azur, Inserm

Exploring Adipose Tissue Functions

Nous utilisons des cookies afin d'améliorer votre expérience sur notre site web.

En continuant à utiliser notre site ou en cliquant sur le bouton ''continuer'', vous acceptez l'utilisation de cookies.

Apprendre encore plus