To begin, place the TuiNa intervened rat on an intelligent cold and hot plate pain detector with a surface temperature of four degrees Celsius. Cover the rat with a transparent plastic cage. Once the rat is acclimatized to the plastic cage, record the number of foot lifts in the operative side of the hind limb for the next five minutes.
Place the rat on a grid surface in a test box for 15 to 30 minutes before testing. Move the probe of the pain to the center of the right posterior plantar region of the rat, and linearly increase the pressure by hand. When the rat lifts or licks its feet, record the threshold displayed on the instrument screen.
Adapt the rat in a glass-bottom test box for 15 minutes before starting the test. On a thermal stimulation pain instrument, click on set and direction buttons to set the cutoff time to 30 seconds, and intensity to 50%Then click on start. Position the infrared probe to the center of the right posterior plantar region of the rat.
When the rat lifts or licks its feet, record the latency of the foot retraction reflex. In cold sensitivity threshold analysis, compared to the model group, the Tuina group showed a significant reduction in foot lifts six hours post-treatment. The model group exhibited a significant increase in foot lifts compared to the sham group, highlighting the effect of the model intervention.
The mechanical withdrawal threshold of each subgroup of Tuina was significantly higher than that of the model group. Except for 18 hours after Tuina, the model group had a significantly lower mechanical withdrawal threshold than the sham operation group. Thermal withdrawal latency analysis revealed a significant increase immediately, six hours, and 18 hours after Tuina in comparison to the model group.
The model group showed a significant decrease compared to the sham operation group.
