Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry

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9.2K Views

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11:02 min

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January 20th, 2017

DOI :

10.3791/55222-v

January 20th, 2017

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Faezzah Baharom¹, Gregory Rankin², Saskia Scholz¹, Jamshid Pourazar², Clas Ahlm³, Anders Blomberg², Anna Smed-Sörensen¹

¹Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet, ²Division of Medicine, Department of Public Health and Clinical Medicine, Umeå University, ³Divison of Infectious Diseases, Department of Clinical Microbiology, Umeå University

Transcription

The main advantage of this technique is that a lot of information can be generated from small tissues obtained during bronchoscopy.This method can help answer key questions in mucosal immunology, where bronchoscopy is part of establishing the diagnosis, such as COPD, sarcoidosis, or lung cancer.Visual demonstration of this procedure will enable individuals new to this method to recognize when enzymatic digestion is complete.Demonstrating this procedure, along with Faezzah Baharom, will be Greg Rankin, a postdoc from our collaborator's lab at Ume

Résumé

Lung-resident immune cells, including dendritic cells (DCs) in humans, are critical for defense against inhaled pathogens and allergens. However, due to the scarcity of human lung tissue, studies are limited. This work presents protocols to process human mucosal endobronchial biopsies for studying lung DCs using immunohistochemistry and flow cytometry.

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