We aim to isolate and cultivate bacteria from the intestine of chickens to improve our knowledge of microbial networks and interactions with the host. The characterization of these anaerobic bacteria supports the knowledge of intestinal processes and enrich bacteria databases for future research. One of the main challenges is the prevention of oxygen exposition and oxygen-reactive species along the sampling and cultivation process to prevent bacterial inactivation.
To our experience, this has improved the diversity recovery of anaerobic bacteria. Our protocol addresses the gap in understanding myo-inositol metabolism in poultry. By isolating potential myo-inositol-metabolizing bacteria from chicken intestine, our goal is to shed light on the specific bacterial community and metabolic pathways involved, advancing knowledge in this area.
Our primary goal is understanding how microbial communities interact with a host, especially in the gut. This is where diet is broken down, nutrients are absorbed, and significant interactions occurs with these microorganisms.
