Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges

Our research describes reproducible methods for assessing the early fate commitment of virus-specific TFH cells, including establishing acute LCMV-infected monoclonal mouse model, conducting flow cytometry staining, and performing retroviral-vector-based gene manipulation. Compared to in-vitro T-cell stimulation, in-vivo stimulation could activate T cells more quickly, even in 12 hours, and it's fully activated for the next retrovirus transduction. Our findings will help in studies exploring the mechanisms underlying the early commitment of various specific TFH cells.

Furthermore, our research will contribute to understanding T-cell-dependent humoral immunity and optimizing vaccine design.