evaluating-the-effect-of-pesticides-on-the-larvae-of-the-solitary-bees

Evaluating the Effect of Pesticides on the Larvae of the Solitary Bees

Current ecological risk assessments of pesticides on pollinators have primarily considered only laboratory conditions. For the larvae of solitary bees, ...

dissection-grading-ovarian-development-wild-type-female

Dissection and Grading of Ovarian Development in Wild-Type Female Insects

Migratory insect pests pose serious challenges to food production and security all over the world. The migratory pests can be monitored and captured using ...

Shandong Academy of Agricultural Sciences

Botryosphaeria dothidea, the causal agent of apple ring rot, is an important fungal plant pathogen that can cause serious reductions in crop yield, and fungicides still play a crucial role in management. In the present study, the sensitivity of B. dothidea to fludioxonil, fluazinam, and pyrisoxazole was assessed in 162 isolates. Moreover, the protective and curative activity of the three fungicides on detached apple fruit as well as the control efficacy in the field were determined. The results showed that the mean 50% effective concentration (EC) values (± standard deviation) were 0.01 ± 0.008, 0.04 ± 0.03, and 0.02 ± 0.01 μg ml, with individual EC values of 0.002 to 0.05, 0.003 to 0.19, and 0.005 to 0.26 μg ml for fludioxonil, fluazinam, and pyrisoxazole, respectively. In addition, the frequency distributions of EC values were both unimodal curves. However, significant correlations (P < 0.05) were found between fludioxonil and iprodione, between fluazinam and iprodione, as well as between pyrisoxazole and difenoconazole. In field trials conducted during 2016 and 2017, the control efficacy ranged from 75.91 to 87.41% when fludioxonil was applied at 100 to 150 mg active ingredient (a.i.) kg, 81.90 to 85.13% when fluazinam was applied at 400 mg a.i. kg, and 77.43 to 80.97% when pyrisoxazole was applied at 400 mg a.i. kg. The control efficacy of the fungicides in storage was higher than 60%, with the exception of fluazinam. These results demonstrated that fludioxonil, fluazinam, and pyrisoxazole have considerable potential to control apple ring rot.

Evaluating the Sensitivity and Efficacy of Fungicides with Different Modes of Action Against Botryosphaeria dothidea.

In this study, the complete mitogenome sequence of  was described. The assembled mitogenome is 15,241 bp in length with an extreme bias of high AT content (80.9%) (GenBank accession no. MN747041). The mitochondrial genome contains 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (12S rRNA and 16S rRNA) genes, and a control region (D-loop region). The mitochondrial gene order was identical to that observed in most lepidopteran genomes, nine PCGs were located on the H-strand, others were located on the L-strand. 12 PCGs were initiated by typical ATN codons, except for  with CGA instead. 21 tRNAs had the typical cloverleaf structure, while the DHU arm of the  gene did not form a stable stem-loop structure. The 'ATAGT(A)'-like motif and a 19 bp poly-T stretch at the down-stream of the  gene were observed in the A + T-rich region. The phylogenetic analysis showed that the relationship of  is very close to the three species in the subfamily Pyraustinae: ,  and , and all the subfamilies of Spilomelinae, Pyraustinae, Crambinae and Nymphulinae within Crambidae formed monophyletic groups with the highest bootstrap value support.

Characterization of the complete mitochondrial genome of Asia Corn Borer,  (Lepidoptera: Crambidae).

Osmia excavata is an important pollinator in commercial fruit orchards. Little information has been published about ecotoxicity to O. excavata, especially the larvae. To clarify the risk of commonly used insecticides with different modes of action to the larvae of O. excavata, six insecticides (clothianidin, acetamiprid, sulfoxaflor, lambda-cyhalothrin, chlorfenapyr and abamectin) were selected for evaluation of their acute lethal toxicity and sublethal effects. Clothianidin and abamectin were the two most toxic insecticides to the larvae of O. excavata with LD values of 0.007 (0.006-0.008) and 0.0004 (0.0003-0.0006) μg active ingredient (a.i.) bee, respectively. And their ecological risks were high according to the hazard quotient values (HQ > 2500). Sulfoxaflor was identified as the only safe insecticide for O. excavata (HQ < 50) under field conditions. Sublethal toxicity tests showed that larval weight was significantly decreased by ingesting food treated with clothianidin, lambda-cyhalothrin and abamectin (less than the maximum field registered concentrations on fruit trees) due to interference with consumption per larva and reduction of the efficiency of conversion of ingested food. Additionally, above three insecticides significantly prolonged larval developmental duration before cocooning and decreased eclosion rate. Overall, there results suggested that clothianidin and abamectin should not be applied, especially during the flowering phase, the application frequency of lambda-cyhalothrin should be minimized for the purpose of conserving O. excavata. Our results provided important evidences for selecting appropriate insecticides for use in fruit orchards.

Comparative ecotoxicity of insecticides with different modes of action to Osmia excavata (Hymenoptera: Megachilidae).

Spodoptera frugiperda is a major agricultural pest that has invaded China since January 2019. Given that most of the individuals present in China carried the diagnostic rice-strain mtDNA (COI-RS), there was no efficient method to distinguish populations of S. frugiperda. In this study, we identified and characterized two variant microsatellite alleles in the mitochondrial NAD6 gene of S. frugiperda retrieved from the National Center for Biotechnology Center GenBank. We then sequenced partial NAD6 genes containing the microsatellite region and the diagnostic COI barcoding gene (used to distinguish the corn-strain and the rice-strain) of 429 invasive S. frugiperda individuals that were collected from the main infested regions in China during 2019-2020. Our data indicates that two kinds of interrupted repeat sequences, (ATA)4T(ATA)3 and (ATA)5T(ATA)3, exist in the microsatellite region which we defined as the deletion type (NAD6-D), and the insertion type (NAD6-I) based on the repeat units' differentiation, respectively. The presence of these two microsatellite types in the mtDNA genome of S. frugiperda was further confirmed with the sequencing results in 429 samples. Moreover, NAD6-I and NAD6-D types were both present in individuals with COI-RS, while only NAD6-D type was detected in the COI-CS individuals. Interestingly, the two microsatellite types suggested a possible geographic distribution: the western migratory route (Yunan and Chongqing) was comprised exclusively of NAD6-I type, while both NAD6-I and NAD6-D types were identified in the predicted eastern migration trajectories (Hainan, Guangxi, Shandong, etc.). These results suggested that NAD6-D and NAD6-I types may be useful in distinguishing between populations, analyzing the evolutionary mechanism of mtDNA microsatellite polymorphism, inferring the migratory route of S. frugiperda in China, and developing precise and integrated control strategies for S. frugiperda.

Two Microsatellite Types Within NAD6 Gene Help to Distinguish Populations and Infer the Migratory Route of the Invasive Fall Armyworm, Spodoptera frugiperda, (Lepidoptera, Noctuidae) in China.

Elevated atmospheric carbon dioxide (eCO) concentrations can alter the carbon:nitrogen ratio and palatability of host plants for herbivorous insects, but rhizobacteria likely mitigate the alteration and influence physiological adaptation of insects. In this study, we conducted transcriptomic analysis of maize (Zea mays) response to Azotobacter chroococcum (AC) inoculation under eCO conditions in contrast to ambient CO (aCO), and studied the effects of plant-defense change of maize under eCO on the oriental armyworm, Mythimna separata. Results showed that there were 16, 14, 16 and 135 differentially expressed genes that were associated with plant-defense response in maize leaves between aCO-CK and aCO-AC, eCO-CK and eCO-AC, aCO-CK and eCO-CK, aCO-AC and eCO-AC, respectively. Moreover, A. chroococcum inoculation and eCO influenced plant hormone signal transduction of maize. Interestingly, A. chroococcum inoculation significantly decreased the contents of JA (jasmonic acid) and JA-Ile (isoleucine conjugate of JA) in leaves, but eCO markedly increased contents of JA-Ile, JA and SA (salicylic acid). Compared to aCO, eCO significantly decreased activity of protective enzyme (catalase), and increased activities of digestive (lipase and protease), protective (peroxidase) and detoxifying enzymes (carboxylesterase, Mixed-functional oxidase and glutathione s-transferase), prolonged developmental time, and decreased survival rate and body weight of larvae (P < 0.05). A. chroococcum inoculation significantly increased the activity of protective enzyme (catalase), and decreased the activities of detoxifying enzymes (carboxylesterase, glutathione s-transferase and mixed-functional oxidase), thus increased the growth rate and body weight of larvae in comparison with no-inoculation of A. chroococcum (P < 0.05). The indices of M. separata were significantly correlated with the foliar contents of JA, JA-Ile and SA (|r| = 0.44-0.85, P < 0.05), indicating that A. chroococcum inoculation altered the physiological adaptation of M. separata under eCO by disturbing defense substances in maize. Our results in understanding effects of A. chroococcum inoculation on maize resistance to herbivorous insects will be valuable for agricultural pest control in the future at eCO conditions.

Yingying Song

Institute of Plant Protection

Shandong Academy of Agricultural Sciences

Evaluating the Effect of Pesticides on the Larvae of the Solitary Bees

Dissection and Grading of Ovarian Development in Wild-Type Female Insects