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Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

12.1K Views

14:02 min

April 21st, 2017

DOI :

10.3791/55674-v

April 21st, 2017


Trascrizione

Esplora Altri Video

Glycoproteomics

Capitoli in questo video

0:05

Title

1:05

Extraction of Extracellular Matrix Proteins

3:52

Protein Precipitation

4:57

Sequential Deglycosylation for the Assessment of N-glycosylation Site Occupancy

6:05

Peptide Cleanup Using C18 Column

8:32

Glycopeptide Enrichment

11:32

Results: Glycopeptide Extraction and Analysis

13:18

Conclusion

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