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Naresuan University
2 ARTICLES PUBLISHED IN JoVE
Biology
Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies
Tim M. Curtis *1, Declan McLaughlin 2, Michael O'Hare 1, Joanna Kur 1, Peter Barabas 1, Gordon Revolta 1, C. Norman Scholfield 3, J. Graham McGeown 4, Mary K. McGahon *2
1Centre for Experimental Medicine, Queen's University of Belfast, 2Centre for Biomedical Sciences (Education), Queen's University of Belfast, 3Department of Pharmaceutical Chemistry and Pharmacognosy, Naresuan University, 4School of Medicine, Dentistry and Biomedical Sciences, Queen's University of Belfast
This manuscript describes a straightforward protocol for the isolation of arterioles from the rat retina that can be used in electrophysiological, calcium imaging and pressure myography studies.
Medicine
Isolation of Intrapulmonary Artery and Smooth Muscle Cells to Investigate Vascular Responses
Kittiwoot To-on 1, Usana Chatturong 1, Teerapap Panklai 2, Iyapa Palang 1, Anjaree Inchan 1, Sutthinee Wisutthathum 3, Tamkeen Urooj Paracha 4, Phapada Apaikawee 1, Krongkarn Chootip 1
1Department of Physiology, Faculty of Medical Science and Center of Excellence for Innovation in Chemistry, Naresuan University, 2Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, 3Cosmetics and Natural Products Research Center, Faculty of Pharmaceutical Sciences, Naresuan University, 4Snyder Institute for Chronic Diseases, Airways Inflammation Research Group, Department of Physiology and Pharmacology, University of Calgary
Vascular responses of arterial pulmonary circulation can be explored using intrapulmonary artery (IPA) and vascular smooth muscle cells (VSMCs). The present study describes the isolation of IPA in detail and the protocols used for investigating vasorelaxation in response to physiological stimuli.
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