サインイン

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by the complete absence of introns. Also, their genes are very closely spaced and some of them even have overlapping regions. D-loop is the most important regulatory non-coding region of mitochondrial DNA, which also contains the origin of the replication for the H-strand. Mitochondrial genetic code differs from nuclear DNA code with respect to a few codons. For example, codon UGA, AUA, and AGA/AGG codes for STOP codon, isoleucine, and arginine, respectively, in nuclear DNA while the same codons codes for tryptophan, methionine and STOP codon, respectively, in animal mitochondrial DNA.

Replication of nuclear DNA is coordinated with the cell cycle and must be finished before cell division occurs. Another characteristic feature of the mitochondrial genome is its relaxed DNA replication, where unlike nuclear DNA, replication is independent of the cell cycle and can go on in daughter cells even after cell division.

Maternal Inheritance

In mammals, mitochondrial DNA gets inherited only from the mother’s oocyte as the mitochondria present in the sperm are selectively degraded by a ubiquitin-mediated pathway in the zygote. Mutations in mitochondrial genes can result in diseases such as Leber’s hereditary optic neuropathy or Leigh syndrome; therefore, if the mother carries such mutations, her offspring can inherit these diseases. Recently, new therapies such as mitochondrial replacement can allow the birth of an unaffected child to an affected mother. The nucleus of the mother’s oocyte is transferred to an enucleated oocyte of a healthy donor with normal mitochondria before fertilization. This technique has led to the birth of the so-called “three-parent baby,” who did not inherit the mother’s mitochondrial disease.

タグ

Animal Mitochondrial GeneticsMitochondriaATP MoleculesOrganellesGenetic SystemAerobic BacteriumSymbiotic AssociationNuclear DNAMitochondrial DNAMuscle CellsRed Blood CellsClosed Circular MoleculeBase Pairs16S RRNA12S RRNATRNAsRespiratory Chain ProteinsCoding RegionsIntronsGenetic Code

章から 6:

article

Now Playing

6.13 : Animal Mitochondrial Genetics

DNA Replication

7.3K 閲覧数

article

6.1 : Replication in Prokaryotes

DNA Replication

50.2K 閲覧数

article

6.2 : Replication in Eukaryotes

DNA Replication

42.8K 閲覧数

article

6.3 : DNA Base Pairing

DNA Replication

26.7K 閲覧数

article

6.4 : The DNA Replication Fork

DNA Replication

35.0K 閲覧数

article

6.5 : Proofreading

DNA Replication

13.4K 閲覧数

article

6.6 : Lagging Strand Synthesis

DNA Replication

48.3K 閲覧数

article

6.7 : DNA Helicases

DNA Replication

20.7K 閲覧数

article

6.8 : The Replisome

DNA Replication

32.1K 閲覧数

article

6.9 : Mismatch Repair

DNA Replication

9.1K 閲覧数

article

6.10 : DNA Topoisomerases

DNA Replication

30.2K 閲覧数

article

6.11 : Telomeres and Telomerase

DNA Replication

22.5K 閲覧数

article

6.12 : Non-nuclear Inheritance

DNA Replication

4.1K 閲覧数

article

6.14 : Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

DNA Replication

11.8K 閲覧数

article

6.15 : Export of Mitochondrial and Chloroplast Genes

DNA Replication

3.6K 閲覧数

See More

JoVE Logo

個人情報保護方針

利用規約

一般データ保護規則

研究

教育

JoVEについて

Copyright © 2023 MyJoVE Corporation. All rights reserved