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Large Insert Environmental Genomic Library Production

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20:59 min

September 23rd, 2009

DOI :

10.3791/1387-v

September 23rd, 2009


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Large Insert Environmental Genomic Library Production

この動画の章

0:00

Title

0:07

Introduction

0:43

End Repair of Sheared Genomic DNA

2:10

Size-Selection of Genomic DNA by Pulsed-Field Gel Electrophoresis (PFGE)

4:48

DNA-recovery by Gel Extraction and Ligation of Recovered DNA to the Fosmid Cloning Vector

10:18

Phage-packaging of Vector-insert Ligation Product and Infection of Phage-Resistant Bacteria

14:30

Plating and Titering

16:34

Preparation for Colony Picking

17:46

Colony Picking, Culturing, and Long-Term Storage

20:32

Conclusion

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