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Neuroscience

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Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence

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08:58 min

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May 4th, 2011

DOI :

May 4th, 2011

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Sava Sakadžić¹, Emmanuel Roussakis², Mohammad A. Yaseen¹, Emiri T. Mandeville³, Vivek J. Srinivasan¹, Ken Arai³, Svetlana Ruvinskaya¹, Weicheng Wu¹, Anna Devor¹^,⁴, Eng H. Lo³, Sergei A. Vinogradov², David A. Boas¹

¹Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, ²Department of Biochemistry and Biophysics, University of Pennsylvania, ³Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, ⁴Departments of Neurosciences and Radiology, University of California

要約

We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.

この動画の章

0:05

Title

0:11

Introduction

0:56

Wide Field of View Imaging of Partial Pressure of Oxygen in Cortical Vasculature of a Rat

4:55

High Resolution Two-photon pO2 Imaging in Cortical Micro Vasculature of a Mouse

8:22

Conclusion

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