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Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

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12:48 min

August 21st, 2017

DOI :

10.3791/55949-v

August 21st, 2017


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Membrane Compartment

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0:05

Title

1:13

Preparation of Microsomes

4:44

Purification of Microsomes with bOVA Underground ERAD

6:59

In Vitro Reconstruction of ERAD Ubiquination of bOVA Using Purified Microsomes

9:04

Results: Purified Microsomes and In Vitro Ubiquination of the Exogenous Antigen

11:06

Conclusion

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