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Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays

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07:15 min

July 22nd, 2022

DOI :

10.3791/64134-v

July 22nd, 2022


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Borreliella Burgdorferi

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0:04

Introduction

0:50

Purification of the RNA Polymerase and Preparation of RNA Polymerase Stock

3:35

In Vitro Transcription with the Incorporation of Radiolabeled Nucleotides

5:35

Results: Analysis of RpoD Purification and In Vitro Transcription

6:43

Conclusion

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