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Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates

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08:32 min

February 17th, 2023

DOI :

10.3791/64241-v

February 17th, 2023


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Intestinal Transcytosis

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0:04

Introduction

0:44

Preparing Transcytosis Inserts with T84 Cells (Approximately 1 - 2 Weeks Before the Experiment)

3:43

Preparing T84 Cells 1 Day Before the Experiment Using TCM w/o Antibiotics and Escherichia coli Cultures (Started 1 Day Before the Experiment)

4:19

Preparing E. coli Inoculum, Epithelial Cells, and Materials (On the Morning of the Experiment)

5:05

Inoculation of the Cells (Start of the Experiment)

5:54

Quantifying the Transcytosis and End of the Experiment

6:56

Results: Transcytosis of Neonatal E. coli Isolates

8:04

Conclusion

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