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Veterans Administration Greater Los Angeles Healthcare System
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina
Allison Sargoy 1, Steven Barnes 1,2,3, Nicholas C. Brecha 1,2,4, Luis Pérez De Sevilla Müller 1
1Department of Neurobiology, University of California, Los Angeles, 2Veterans Administration Greater Los Angeles Healthcare System, 3Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences, Dalhousie University, 4Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine, University of California, Los Angeles
Immunohistochemistry protocols are used to study the localization of a specific protein in the retina. Calcium imaging techniques are employed to study calcium dynamics in retinal ganglion cells and their axons.
Biology
Specimen Collection and Analysis of the Duodenal Microbiome
Benjamin W. Dreskin 1, Kayti Luu 1, Tien S. Dong 1,2,3, Jihane Benhammou 1,2,4, Venu Lagishetty 2,3, John Vu 1, Daniel Sanford 1, Francisco Durazo 2,4, Vatche G. Agopian 4, Jonathan P. Jacobs 1,2,3, Joseph R. Pisegna 1,5, Shehnaz K. Hussain 6
1Division of Gastroenterology, Hepatology and Parenteral Nutrition, Veterans Administration Greater Los Angeles Healthcare System, 2The Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at University of California, 3UCLA Microbiome Center, David Geffen School of Medicine at University of California, 4Department of Surgery, University of California, Los Angeles, 5Department of Medicine and Human Genetics, David Geffen School of Medicine at University of California, 6Cedars-Sinai Cancer and Department of Medicine, Cedars-Sinai Medical Center
In this manuscript, we discuss a novel method to sample and analyze the duodenal microbiome. This method provides an accurate depiction of microbial diversity and composition in the duodenum and could be useful for further investigation of the duodenal microbiome.
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