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The Multifaceted Benefits of Protein Co-expression in Escherichia coli

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12:48 min

February 5th, 2015

DOI :

10.3791/52431-v

February 5th, 2015


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Protein Co expression

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0:05

Title

2:11

Isolation of E. coli Co-transformants

4:19

Co-expression of α and ε Subunits of E. coli DNA Polymerase III

6:20

Co-expression of α Subunit of Deinococcus radiodurans DNA Polymerase III and ε Subunit of E. coli DNA Polymerase III

6:49

Gel Filtration Chromatography

8:09

Mutation Analysis of Populations Co-expressing the Wild-type ε Subunit of E. coli DNA Polymerase III and the Mutagenic εD12A Variant

10:43

Results: Effective Protein Co-expression in E. coli

11:55

Conclusion

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