Brigham and Women's Hospital-Harvard Medical School

1 ARTICLES PUBLISHED IN JoVE

Genetics

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Vivek Bhaskaran¹, Pierpaolo Peruzzi¹

¹Department of Neurosurgery, Brigham and Women's Hospital-Harvard Medical School

Described here is a protocol for characterizing modules of biologically synergistic miRNAs and their assembly into short transgenes, which allows simultaneous overexpression for gene therapy applications.