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An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes

4.7K Views

08:23 min

December 25th, 2021

DOI :

10.3791/62623-v

December 25th, 2021


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Automated Microscopic Scoring H2AX Foci Assay Human Peripheral Blood Lymphocytes Ionizing Radiation DNA Double strand Breaks H2AX Phosphorylation Automated Slide Scanning Fluorescent Microscopy Lymphocyte Isolation Density Gradient Centrifugation Gamma Irradiation Cytocentrifugation Standardized Slide Preparation

Rozdziały w tym wideo

0:00

Introduction

0:44

Preparation of Samples

1:26

Sample Irradiations

1:35

Slide Preparation

2:17

Fixation and Immunofluorescence γ-H2AX Staining

3:44

Automated Metafer Scanning and Scoring

7:29

Results

8:03

Conclusion

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