Measurement of Glutamate Uptake using Radiolabeled L-[3H]-Glutamate in Acute Transverse Slices Obtained from Rodent Resected HippocampusDébora G. Souza 1, Yasmine Nonose 1, Diogo O. Souza 1,2, Roberto F. Almeida 3,4
1Graduate Program in Biochemistry, Universidade Federal do Rio Grande do Sul, 2Department of Biochemistry, Universidade Federal do Rio Grande do Sul, 3Department of Biological Sciences, Universidade Federal de Ouro Preto, 4Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal de Ouro Preto
This article describes a reliable and simple way to obtain ex vivo acute hippocampal transverse slices from mice and rats using a tissue chopper. Slices obtained from resected hippocampi can be submitted for functional glutamate uptake analysis to investigate glutamatergic system homeostasis.
