JOVE JOURNAL

Developmental Biology

Showing 1 - 12 in 811 Videos

Combining ilastik and Flynotyper for Accurate Detection and Quantification of Ommatidia in <i>Drosophila melanogaster </i> Fly Eye Images

Journal (Developmental Biology)

Combining ilastik and Flynotyper for Accurate Detection and Quantification of Ommatidia in Drosophila melanogaster Fly Eye Images

Qasim Mujteba*¹, Madeleine R. Chalmers*¹, JiHye Kim¹, Minwoo Baek¹, Nam Chul Kim¹

¹Department of Pharmacy Practice and Pharmaceutical Sciences, College of Pharmacy, University of Minnesota

High-Resolution Cell Transplantation in Zebrafish for Nerve Development Studies

Journal (Developmental Biology)

High-Resolution Cell Transplantation in Zebrafish for Nerve Development Studies

Lindsey S. Qian¹, Rabab Ibrahim¹^,², Adam J. Isabella¹^,²

¹Department of Genetics, Cell Biology, and Development, University of Minnesota, ²Graduate Program in Molecular, Cellular, Developmental Biology and Genetics, University of Minnesota

Preparing Donor Cells and Host Zebrafish for Cell Transplantation to Study Developmental Mechanisms

Journal (Developmental Biology)

Preparing Donor Cells and Host Zebrafish for Cell Transplantation to Study Developmental Mechanisms

Lindsey S. Qian¹, Rabab Ibrahim¹^,², Adam J. Isabella¹^,²

¹Department of Genetics, Cell Biology, and Development, University of Minnesota, ²Graduate Program in Molecular, Cellular, Developmental Biology and Genetics, University of Minnesota

Quantifying <i>Drosophila melanogaster</i> Eye Phenotypes: A Computational Approach Integrating ilastik and Flynotyper

Journal (Developmental Biology) | Methods Collections

Quantifying Drosophila melanogaster Eye Phenotypes: A Computational Approach Integrating ilastik and Flynotyper

Qasim Mujteba*¹, Madeleine R. Chalmers*¹, JiHye Kim¹, Minwoo Baek¹, Nam Chul Kim¹

¹Department of Pharmacy Practice and Pharmaceutical Sciences, College of Pharmacy, University of Minnesota

Same-Day Genotyping of Isolated Gastrulating Murine Embryos

Journal (Developmental Biology)

Same-Day Genotyping of Isolated Gastrulating Murine Embryos

Elizabeth Abraham¹, Mikel Zubillaga¹, Thomas Roule², Eleonora Stronati³, Naiara Akizu², Conchi Estaras¹

¹Department of Cardiovascular Sciences, Aging + Cardiovascular Discovery Center, Temple University, Lewis Katz School of Medicine, ²Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, ³Department of Child and Adolescence Psychiatry, Children's Hospital of Philadelphia

Isolation of Murine Embryos During Gastrulation for Single-Cell RNA Sequencing

Journal (Developmental Biology)

Isolation of Murine Embryos During Gastrulation for Single-Cell RNA Sequencing

Elizabeth Abraham¹, Mikel Zubillaga¹, Thomas Roule², Eleonora Stronati³, Naiara Akizu², Conchi Estaras¹

¹Department of Cardiovascular Sciences, Aging + Cardiovascular Discovery Center, Temple University, Lewis Katz School of Medicine, ²Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, ³Department of Child and Adolescence Psychiatry, Children's Hospital of Philadelphia

Cell Dissociation of Isolated Gastrulating Murine Embryos for Single-Cell Sequencing

Journal (Developmental Biology)

Cell Dissociation of Isolated Gastrulating Murine Embryos for Single-Cell Sequencing

Elizabeth Abraham¹, Mikel Zubillaga¹, Thomas Roule², Eleonora Stronati³, Naiara Akizu², Conchi Estaras¹

¹Department of Cardiovascular Sciences, Aging + Cardiovascular Discovery Center, Temple University, Lewis Katz School of Medicine, ²Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, ³Department of Child and Adolescence Psychiatry, Children's Hospital of Philadelphia

Cellular Fractionation of Rhesus Macaque Pancreatic Islets for Single-Cell RNA Sequencing and Metabolomics

Journal (Developmental Biology)

Cellular Fractionation of Rhesus Macaque Pancreatic Islets for Single-Cell RNA Sequencing and Metabolomics

Darian T. Carroll¹, Sarah R. Lindsley², Melissa Kirigiti², Alex Buko³, Angela Jones⁴, Paul Kievit², Maureen Gannon¹^,⁵^,⁶^,⁷

¹Department of Molecular Physiology and Biophysics, Vanderbilt University, ²Division of Metabolic Health and Disease, Oregon National Primate Research Center, ³Human Metabolome Technologies (HMT) America, ⁴Vanderbilt Technologies for Advanced Genomics, Vanderbilt University Medical Center, ⁵Department of Medicine, Vanderbilt University Medical Center, ⁶Department of Veterans Affairs Tennessee Valley, ⁷Department of Cell and Developmental Biology, Vanderbilt University

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

Journal (Developmental Biology) | Methods Collections

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

Lindsey S. Qian¹, Rabab Ibrahim¹^,², Adam J. Isabella¹^,²

¹Department of Genetics, Cell Biology, and Development, University of Minnesota, ²Graduate Program in Molecular, Cellular, Developmental Biology and Genetics, University of Minnesota

Extraction and Electroporation of Mouse Embryo Heart as a Transgenesis Approach

Journal (Developmental Biology)

Extraction and Electroporation of Mouse Embryo Heart as a Transgenesis Approach

Jorge Mañes-García¹, Leonardo Beccari¹, Miguel Torres²^,³, Oscar H. Ocaña²^,⁴

¹Tissue and Organ Homeostasis Program, Severo Ochoa Center for Molecular Biology (CBMSO), ²Cardiovascular Regeneration Program, National Cardiovascular Research Center (CNIC), ³Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), ⁴Department of Experimental Biology, Faculty of Experimental Sciences, University of Jaén

Electroporated Mouse Cardiac Cell Isolation for Cell Sorting and Analysis

Journal (Developmental Biology)

Electroporated Mouse Cardiac Cell Isolation for Cell Sorting and Analysis

Jorge Mañes-García¹, Leonardo Beccari¹, Miguel Torres²^,³, Oscar H. Ocaña²^,⁴

¹Tissue and Organ Homeostasis Program, Severo Ochoa Center for Molecular Biology (CBMSO), ²Cardiovascular Regeneration Program, National Cardiovascular Research Center (CNIC), ³Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), ⁴Department of Experimental Biology, Faculty of Experimental Sciences, University of Jaén

Isolation of Granulosa Cell-Free Stage I Oocytes from Zebrafish Ovaries

Journal (Developmental Biology)

Isolation of Granulosa Cell-Free Stage I Oocytes from Zebrafish Ovaries

Qianwen Zheng¹, Xiaolong Xie¹, Yao Li¹, Chengbo Ai¹, Siyu Pu¹, Jing Chen¹

¹Department of Pediatric Surgery and Laboratory of Pediatric Surgery, West China Hospital / West China School of Medicine, Sichuan University

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