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Norwich Research Park
2 ARTICLES PUBLISHED IN JoVE
Biology
Identification of Post-translational Modifications of Plant Protein Complexes
Sophie J. M. Piquerez 1, Alexi L. Balmuth 2, Jan Sklenář 2, Alexandra M.E. Jones 1,2, John P. Rathjen 3, Vardis Ntoukakis 1
1School of Life Sciences, University of Warwick, 2The Sainsbury Laboratory, Norwich Research Park, 3Research School of Biology, The Australian National University
We describe here a protocol for the purification and characterization of plant protein complexes. We demonstrate that by immunoprecipitating a single protein within a complex, so we can identify its post-translational modifications and its interacting partners.
Genetics
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Joanna M. Feehan *1,2, Katherine E. Scheibel *1, Salim Bourras 3, William Underwood 4, Beat Keller 3, Shauna C. Somerville 1
1Department of Plant and Microbial Biology, University of California Berkeley, 2John Innes Centre, Norwich Research Park, 3Department of Plant and Microbial Biology, University of Zürich, 4USDA-ARS Sunflower and Plant Biology Research Unit
Described here is a method for the extraction, purification, and quality control of genomic DNA from the obligate biotrophic fungal pathogen, powdery mildew, for use in long-read genome sequencing.
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