Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

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Introduction

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Incubation of eggs

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Ex ovo culture

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Application of substances for ex ovo cam assay

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Inoculation of cells onto the CAM

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Intravitreous injection of cells

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Dissection and grafting of chicken limb buds

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Preparation and grafting of mouse limb buds

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Grafting of tumor samples

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Re-grafting of tumor samples

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Conclusion