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Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein

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11:01 min

March 31st, 2010

DOI :

10.3791/1874-v

March 31st, 2010


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Stopped flow Kinetics Methods

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0:00

Title

0:57

Introduction

1:40

Sample Preparation for the Msh2-Msh6/ DNA Binding Experiment

3:07

Instrument Preparation for the Msh-2Msh6/DNA Binding Experiment

5:07

Msh2-Msh6/DNA Binding Experiment

6:27

Representative Results for the Msh2-Msh6/DNA Binding Kinietics Experiment

7:00

Sample and Instrument Preparation for the Msh2-Msh6 ATPase Experiment

8:30

Msh2-Msh6 ATPase Experiment

9:40

Representative Results for Msh2-Msh6 ATPase Kinetics Experiments

10:20

Conclusion

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