Quantification of γH2AX Foci in Response to Ionising Radiation

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20.3K Views

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06:53 min

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April 6th, 2010

DOI :

10.3791/1957-v

April 6th, 2010

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Li-Jeen Mah¹^,², Raja S. Vasireddy¹^,²^,³, Michelle M. Tang¹^,³, George T. Georgiadis¹, Assam El-Osta²^,³, Tom C. Karagiannis¹^,²

¹Epigenomic Medicine, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, ²Department of Pathology, The University of Melbourne, ³Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct

Резюме

Quantification of DNA double-strand streaks using γH2AX formation as a molecular marker has become an invaluable tool in radiation biology. Here we demonstrate the use of an immunofluorescence assay for quantification of γH2AX foci after exposure of cells to radiation.

Смотреть дополнительные видео

H2AX Foci

Quantification

Ionising Radiation

DNA Double strand Breaks

H2A Histone Variant

Phosphorylation

Serine 139 Residue

C terminal SQEY Motif

Phosphatidyl inosito 3 kinase PIKK Family

Ataxia Telangiectasia Mutated ATM

DNA protein Kinase Catalytic Subunit