Begin by setting up the ladder assembly for the Ladder Beam Test. After connecting the four parallel plexiglass containing the holes, proceed to design the various kinds of ladder rungs by inserting aluminum sticks into the holes on the plexiglass. For the Horizontal Ladder Test insert aluminum sticks into the holes on the plexiglass walls.
For the Zigzag Ladder Test, insert the aluminum sticks into every third hole in the horizontal, and vertical directions on the plexiglass walls. In both cases, adjust the widths of the ladder rungs as required by widening or narrowing the distance of the plexiglass walls. Then place the dark chamber in the target site to motivate the animals to move toward the goal.
Finally, place the cushion under the ladder rungs for safety.
