To begin, plate 10 microliters of the mother stock suspension of Trichoderma stromaticum on the PDA plate. Incubate the culture at 28 degrees Celsius in the dark for seven to 10 days until the conidia are observed and the culture turns green to obtain the fresh F1 generation. Wash the culture by adding three to five milliliters of PBS to the plate, then collect and again dispense the PBS over the culture to gradually remove the conidia.
Repeat this process until the suspension turns dark green. Transfer the recovered suspension from the plate into a sterile 15-milliliter tube. Centrifuge the suspension at 1, 160 G for five minutes at 12 degrees Celsius and resuspend the pellet in five milliliters of PBS.
Resuspend the final pellet in two to three milliliters of PBS and count the conidia in a Neubauer chamber.
