analysis of single-molecule imaging data to study interactions of halo-tagged proteins

Live Cell Single-Molecule Imaging-Based Quantification of Protein Interactions

A growing body of work suggests that biomolecular condensates play an important role in cellular organization and numerous cellular functions, e.g., transcriptional regulation1,2,3,4,5, DNA damage repair6,7,8, chromatin organization9,10,11,12, X-chromosome inactivation13,14,15, and intracellular signaling16,17,18. In addition, the dysregulation of biomolecular condensates is implicated in many diseases, including cancers19,20,21 and neurodegenerative disorders22,23,24,25,26. Condensate formation is often driven by transient, selective, and multivalent protein-protein, protein-nucleic acid, or nucleic acid-nucleic acid interactions27. Under certain conditions, these interactions can lead to liquid-liquid phase separation (LLPS), a density transition that locally enriches specific biomolecules in membraneless droplets. Such multivalent interactions are often mediated by the intrinsically disordered regions (IDRs) of proteins1,28,29. Biophysical characterization of these interactions at the molecular level is critical to our understanding of numerous healthy and aberrant cellular functions, given the pervasiveness of condensates across them. Although techniques based on confocal fluorescence microscopy, e.g., fluorescence recovery after photobleaching (FRAP)30,31,32, have been widely used to qualitatively show that the molecular exchanges between condensates and the surrounding cellular environment are dynamic, quantifying the interaction dynamics of specific biomolecules within condensates is generally not possible using conventional confocal microscopy or single-molecule microscopy without specialized data analysis methods. The single-particle tracking (SPT) technique described in this protocol is based on live-cell single-molecule microscopy33 and provides a uniquely powerful tool to quantify the interaction dynamics between specific proteins within condensates. The readout of SPT for such measurement is the mean residence time of a protein of interest in the condensates.
The protocol can be broken down into two parts - data acquisition and data analysis. The first step of imaging data acquisition is to express in cells a protein of interest that is fused to a HaloTag34. This enables labeling of the protein of interest with two fluorophores, where a majority of the protein molecules are to be labeled with a non-photoactivatable fluorophore (e.g., JFX549 Halo ligand35) and a small fraction of them are to be labeled with a spectrally distinct, photoactivatable fluorophore (e.g., PA-JF646 Halo ligand36). This allows for the simultaneous acquisition of all condensate locations in the cell and the acquisition of single-molecule movies of the protein of interest binding and unbinding to the condensates. Meanwhile, the same type of cells are modified to stably express Halo-tagged H2B, a histone that is largely immobile on chromatin. The cells are then stained with the PA-JF646 Halo ligand to enable single-molecule imaging of H2B. As will be discussed in detail below, this experiment accounts for the contribution of photobleaching to enable precise quantification of the interaction dynamics of the protein of interest. Cells for imaging experiments must then be cultured on clean coverslips, stained with HaloTag ligand(s), and assembled into a live-cell imaging chamber. From there, the sample is imaged under highly inclined and laminated optical sheet (HILO) illumination on a total internal reflection fluorescence (TIRF) microscope capable of two-channel imaging and single-molecule detection. The emission is then split onto two cameras, one tracking condensate positions and one tracking single molecules. Acquisition is performed with a long integration time (on the order of hundreds of ms) to blur out freely-diffusing proteins and only capture proteins that are less mobile due to binding to stable structures in the cell37.
The first step of data analysis is using an established single-particle tracking (SPT) algorithm38,39 to localize individual protein molecules in each frame of the movie and assemble the localizations into a trajectory for each molecule over its detectable lifetime. The trajectories are then sorted into those representing molecules inside and those representing molecules outside the condensates by comparing the localizations of the molecules throughout their trajectories to the localizations of all the condensates at the corresponding times1.
Next, a survival curve (1 - CDF) is generated using the lengths of all the in-condensate trajectories. The apparent mean residence time of the molecules is then extracted by fitting the survival curve to the following two-component exponential model of protein binding,
<img align="center" alt="Equation 1" src="/files/ftp_upload/66169/66169eq01.jpg" style="margin: auto;" />,
with A as the fraction of molecules non-specifically bound and with kobs,ns and kobs,s as the observed dissociation rates of the non-specifically bound and specifically bound molecules, respectively. Only kobs,s is considered from here onward. The dynamics of both protein dissociation, ktrue,s, and photobleaching of the fluorophore, kpb, contribute to kobs,s as
<img alt="Equation 2" src="/files/ftp_upload/66169/66169eq02.jpg" style="margin: auto;" />;
thus, to isolate the effects of protein dissociation, the specific dissociation rate of H2B-Halo in the cell line mentioned prior is measured.
<img alt="Equation 3" src="/files/ftp_upload/66169/66169eq03.jpg" style="margin: auto;" />
H2B is a protein that is stably integrated into chromatin and that experiences minimal dissociation in the time scale of a single-molecule movie acquisition37. Its specific dissociation rate is then equal to the photobleaching rate of the PA-JF646 Halo ligand, or
<img alt="Equation 4" src="/files/ftp_upload/66169/66169eq04.jpg" style="margin: auto;" />.
The mean in-condensate residence time of the protein of interest, <img alt="Equation 5" src="/files/ftp_upload/66169/66169eq05.jpg" style="margin: auto;" />, is then
<img alt="Equation 6" src="/files/ftp_upload/66169/66169eq06.jpg" style="margin: auto;" />.
Representative results from Irgen-Gioro et al.40 are shown, where this protocol was applied to demonstrate that fusing an oligomerization domain to IDR results in longer residence times of the IDR in its condensates. This result suggests that the added oligomerization domain stabilizes the homotypic interactions of the IDR that drives LLPS. In principle, the same method with slightly modified protocols can be applied to characterize the homotypic or heterotypic interactions of any protein that participates in the formation of any types of condensates.

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates

Analysis of Single-Molecule Imaging Data to Study Interactions of Halo-Tagged Proteins

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Research

JoVE Journal

Biology

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Biomolecular condensates formed via liquid-liquid phase separation (LLPS) have been considered critical in cellular organization and an increasing number of cellular functions. Characterizing LLPS in live cells is also important because aberrant condensation has been linked to numerous diseases, including cancers and neurodegenerative disorders. LLPS is often driven by selective, transient, and multivalent interactions between intrinsically disordered proteins. Of great interest are the interaction dynamics of proteins participating in LLPS, which are well-summarized by measurements of their binding residence time (RT), that is, the amount of time they spend bound within condensates. Here, we present a method based on live-cell single-molecule imaging that allows us to measure the mean RT of a specific protein within condensates. We simultaneously visualize individual protein molecules and the condensates with which they associate, use single-particle tracking (SPT) to plot single-molecule trajectories, and then fit the trajectories to a model of protein-droplet binding to extract the mean RT of the protein. Finally, we show representative results where this single-molecule imaging method was applied to compare the mean RTs of a protein at its LLPS condensates when fused and unfused to an oligomerizing domain. This protocol is broadly applicable to measuring the interaction dynamics of any protein that participates in LLPS.

Many intrinsically disordered proteins have been shown to participate in the formation of highly dynamic biomolecular condensates, a behavior important for numerous cellular processes. Here, we present a single-molecule imaging-based method for quantifying the dynamics by which proteins interact with each other in biomolecular condensates in live cells.

Biomolecular condensates formed via liquid-liquid phase separation (LLPS) have been considered critical in cellular organization and an increasing number ...