Application of the Differential Transformation for Cross-Device Co-Registration of Two Preclinical Imaging Modalities

To begin, launch the analysis software. Import the CT file as an underlay by clicking on file underlay and load underlay. In the ensuing dialogue, select the CT image file to open it.

Load the PET file as an overlay by sequentially selecting file overlay and load overlay. In the dialogue that appears, choose the PET image file. To enable the display of both layers, go to menu view followed by layer visibility and check both underlay and overlay.

To load and apply the previously saved differential transformation matrix, click on fusion overlay transformation and then load and apply transform. Then select the file containing the matrix from the calibration process to open it.