To begin, add 70%ethanol to the chamber of the biolistic particle delivery system. After 15 minutes, wipe the ethanol with a clean paper towel. Open the helium tank valves to set a pressure of 2, 000 pounds per square inch.
Next, use a pair of sterile forceps to place a rupture disc on the retaining cap, and screw the retaining cap on the acceleration tube. Use a pair of forceps to place a macrocarrier facing down on the macrocarrier holder, then screw the macrocarrier lid on top of the macrocarrier. Place the microcarrier launch system inside the vacuum chamber at the fifth level from the bottom.
Then, place a plate containing the rho zero strain lawn on the second level from the bottom of the chamber. Put the vacuum vent hold switch in the vacuum position. When the vacuum reaches 20 to 21 inches of mercury, change the vacuum vent hold switch to the hold position.
Press the fire switch to shoot the tungsten particles. When the pressure reaches 1, 300 pounds per square inch, the rupture disc will break, making a pop sound. Immediately after the rupture disc breaks, change the vacuum vent hold switch to the vent position to release the fire switch and the vacuum.
Use sterile forceps to carefully remove any debris from the rupture disc present on the surface of the plate. Then cover the plate with its lid.
