Assessing the Effect of Oleic Acid on HepG2 Cell Viability Using Cell Counting Kit-8

To begin seed overnight-grown culture of HepG2 in 100 microliters of DMEM in each well of a 96-well plate. Incubate the cells at 37 degrees Celsius and 5%carbon dioxide for 24 hours. The next day discard the supernatant and add 100 microliters of oleic acid per well according to the specified grouping.

For the control group, add 100 microliters of DMEM to each well. Incubate the cells at 37 degrees Celsius for 24 hours. After incubation, add 10 microliters of cell counting reagent to each well, mix gently, and incubate for two hours in the dark.

Place the plate in the microplate reader and measure the absorbance at 450 nanometers. Calculate the GI50 values of HepG2 cells according to the optical density. Compared to the control, oleic acid decreased HepG2 cell survival rates at concentrations of 0.125, 0.25, 0.5, and one millimolar, with statistical significance observed at 0.5 and one millimolar.