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PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

53.3K Views

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12:24 min

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July 2nd, 2010

DOI :

10.3791/2034-v

July 2nd, 2010

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1Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Rockefeller University, 2Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, 3Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 4Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 5Genomics Resource Center, Rockefeller University

Смотреть дополнительные видео

PAR CliP
Transcriptome wide
Binding Sites
RNA binding Proteins
Post transcriptional Gene Regulation
RNA Transcripts
RNA RBP Interactions
MicroRNA containing Ribonucleoprotein Complexes
Cell type Dependently
Interplay
High Resolution Maps
In Vivo Protein RNA Interactions
Genetic Approaches
Biochemical Approaches
Computational Approaches
Microarray Profiling
RIP Chip
Kinetically Stable Interactions
RBP Recognition Element RRE
Direct RBP Target Site Information
UV Crosslinking
Immunoprecipitation
CLIP
UV 254 Nm RNA protein Crosslinking

Главы в этом видео

0:00

Title

0:53

Introduction

1:27

In vivo UV Crosslinking

4:06

Immunoprecipitation of Protein

7:07

Recovery of Crosslinked RNA-Protein Segments

10:19

Representative PAR-CLiP Results

12:01

Conclusion

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