Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using ³¹P NMR Spectroscopy

Langendorff-mode isolated heart perfusion, in conjunction with ³¹P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.