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A Gradient-generating Microfluidic Device for Cell Biology

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15.2K Views

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11:05 min

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August 30th, 2007

DOI :

10.3791/271-v

August 30th, 2007

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Bong Geun Chung¹, Amir Manbachi¹, Wajeeh Saadi¹, Francis Lin¹, Noo Li Jeon¹, Ali Khademhosseini¹

¹Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital

Резюме

Смотреть дополнительные видео

Gradient generating Microfluidic Device

Cell Biology

Cellular Behavior

Microfluidic Platform

Fluid Flows

High throughput Experiments

Soluble Concentration Gradients

PDMS based Microfluidic Devices

Growth Factors

Fluorescein Isothyiocyanate FITC dextran

Epidermal Growth Factor EGF

Fibroblasts

Cell Migration

Cell Motility

Cell Tracking Analysis

Главы в этом видео

0:05

Introduction

0:21

Microfabrication of microfluidic device

0:56

Preparation of PDMS mixture

1:49

Patterning PDMS molds

2:20

Removing bubbles by vacuum

3:19

Assembly of microfluidic device

3:20

Cutting layers of microfluidic device

3:33

Creating channel inlet and outlet

3:59

Bonding the microfluidic device layers

6:55

Experiment: effect of EGF gradient on cell migration

7:13

Application of reagents

9:49

Experiment: cell migration

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