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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

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43.4K Views

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10:21 min

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September 21st, 2011

DOI :

10.3791/3060-v

September 21st, 2011

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Patrick J. M. Murphy¹, Orrin J. Stone², Michelle E. Anderson¹

¹College of Nursing, Interdisciplinary Life Sciences Research Laboratory, Seattle University, ²College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory, Seattle University

Резюме

Смотреть дополнительные видео

Automated Hydrophobic Interaction Chromatography

Protein Purification

Chromatographic Medium Selection

Scouting

Hydrophobicity

Affinity Chromatography

Ion Exchange Chromatography

Protein Concentration

Ammonium Sulfate

Purification Method

Nonpolar Ligand

Chromatography Column

Salt Concentration

Главы в этом видео

0:05

Title

1:30

Buffer and Sample Preparation

2:48

Physical Setup and Plumbing of the DuoFlow Chromatography System

4:11

Priming System Lines,Programming Run Method, and Equilibrating HIC Columns

5:43

Sample Loading, Programming the Software, and Running the Column Scouting Protocol

7:36

Representative Chromatogram and Fluorescent Fraction Data

9:23

Conclusion

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