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A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

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13.4K Views

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14:39 min

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November 28th, 2011

DOI :

10.3791/3148-v

November 28th, 2011

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Michael Winkler¹, Nancy Trieu², Tao Feng², Liang Jin², Stephanie Walker², Lipi Singh², Hsun Teresa Ku²^,³

¹Department of Biotechnology & Bioinformatics, California State University Channel Islands, ²Department of Diabetes, Endocrinology and Metabolism, Beckman Research Institute of City of Hope, ³The Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope

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Quantitative Assay

Insulin expressing Colony forming Progenitors

Pancreatic Stem Cells

Progenitor Cell Biology

Single Cell Deposition

Culture Media

Numeration

Labor intensive

Pancreatosphere initiating Progenitors

Proliferation And Differentiation

Semi solid Media Based Colony Assay

Methylcellulose

Three dimensional Space

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0:05

Title

3:34

Generating Conditioned Medium (CM) For the Colony Assay

5:13

Obtaining a Single Cell Suspension of Pancreatic-like Progenitors by Fluorescence Activated Cell Sorting

7:55

Plating Sorted Cells to Semi-solid Culture

10:49

Observing Colony Formation under the Light Microscope

11:47

Ngn3-EGFP+ Derived Colonies Express Insulin

13:21

Conclusion

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