Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

1:57

Preparation of Recombinant MP-12 Encoding NSs Mutations

3:38

Amplification of P0 Virus

4:16

Titration of Recombinant MP-12 by Plaque Assay

6:01

Screening of NSs Mutants Lacking the Type-I IFN Suppression Function

7:03

Reverse Genetics Approach to the C13type MP-12 Virus

8:22

Conclusion