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JoVE Journal

Neuroscience

This content is Open Access.

Derivation of Glial Restricted Precursors from E13 mice

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11.2K Views

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08:56 min

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June 20th, 2012

DOI :

10.3791/3462-v

June 20th, 2012

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André W. Phillips¹^,², Sina Falahati¹^,², Roshi DeSilva¹^,³, Irina Shats², Joel Marx¹, Edwin Arauz¹, Douglas A. Kerr⁴, Jeffrey D. Rothstein²^,⁵, Michael V. Johnston¹^,²^,⁶, Ali Fatemi¹^,²^,⁶

¹Hugo W. Moser Research Institute at Kennedy Krieger, Johns Hopkins University, ²Department of Neurology, Johns Hopkins School of Medicine, ³University of Maryland , ⁴Experimental Neurology, Biogen Idec, ⁵The Brain Science Institute, Johns Hopkins School of Medicine, ⁶Department of Pediatrics, Johns Hopkins School of Medicine

Резюме

This protocol outlines the derivation of Glial Restricted Precursors from fetal spinal cords and maintained in vitro either for transplantation or for the study of oligodendrocytic lineage.

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Glial Restricted Precursor

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Oligodendrocyte Progenitor Cells

OPC

Immature Oligodendrocytes

Glutamate Excitotoxicity

Oligodendrocyte Progenitors

Transplantation Experiments

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