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Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

16.0K Views

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12:03 min

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February 21st, 2012

DOI :

10.3791/3466-v

February 21st, 2012

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1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Center for Neural Development and Disease, University of Rochester Medical Center, 3Deptartment of Neurology, Center for Neural Development and Disease, University of Rochester Medical Center

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Detection
Microregional Hypoxia
Mouse Cerebral Cortex
Two photon Imaging
Endogenous NADH Fluorescence
Oxygen Supply
Blood Flow
Tissue Oxygen Diffusion
Visualized Experimental Protocol
Methodological Protocol
Perivascular Oxygen Gradients
Non linear Relationship
Nicotinamide Adenine Dinucleotide NADH
Fluorescence Intensity
Oxygen Partial Pressure
Two photon Excitation
Intrinsic NADH Tissue Fluorescence
Texas Red Dextran
Advantages
Standard Two photon In Vivo Imaging Equipment
Continuous Monitoring
Depth Resolution
Vulnerable Watershed Areas
Decline In Vascular Oxygen Supply
Microregional Cortical Oxygenation

Главы в этом видео

0:05

Title

1:42

Preparing the Animal for IMaging

2:56

Preparing the Open Skull Cranial Window

6:40

Intravenous Injection and Blood Oxygenation Monitoring

7:58

Two-photon Imaging

9:30

Representative Hypoxia Measurement Results

11:14

Conclusion

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