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Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

11.5K Views

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16:49 min

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July 16th, 2012

DOI :

10.3791/4029-v

July 16th, 2012

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1Division of Experimental Virology, Twincore, Centre for Experimental and Clinical Infection Research, 2Aaron Diamond AIDS Research Center, Laboratory of Retrovirology, The Rockefeller University, NY

Смотреть дополнительные видео

Heterokaryon Formation
HCV Restriction Factors
HCV Replication
Host range
Chimpanzees
HCV RNA Replication
Non hepatic Cell Lines
Murine Cell Lines
Selection Procedures
HCV Replicon Constructs
Antibiotic selectable Markers
In Vitro Research
Hepatoma Cell Lines
Viral Life Cycle
Species Tropism
Immunocompetent Small Animal Model
Genetic Incompatibility
Host Dependency Factors
Restriction Factors
Conditional Trans complementation Methods
Somatic Cell Fusion
Viral Replication Cycle
Heterokaryons
Trans complementation
Infectious Viral Progeny
Subgenomic HCV Rep

Главы в этом видео

0:05

Title

2:02

Induction of Fusion by PEG

5:04

Immunofluorescence to Determine Fusion Efficiency

7:21

Co-culture and Transfection of a Fusogenic PFV Glycoprotein

8:24

Infection Assay

9:33

Visualization of Cell Fusion

10:56

Results: Using Heterokaryon Formation to Assess the Influence of Dominant Restrictions on the HCV Life Cycle

16:23

Conclusion

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