Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

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13:06 min

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October 7th, 2012

DOI :

10.3791/4073-v

October 7th, 2012

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Elena Ronander¹^,², Dominique C. Bengtsson¹^,², Louise Joergensen¹^,², Anja T. R. Jensen¹^,², David E. Arnot¹^,²^,³

¹Centre for Medical Parasitology, Department of International Health, Immunology & Microbiology, Faculty of Health Sciences, University of Copenhagen, ²Department of Infectious Diseases, Copenhagen University Hospital (Rigshospitalet), ³Institute of Infection and Immunology Research, School of Biology, University of Edinburgh

Резюме

Fluorescent in situ hybridization (FISH) to identify mRNA transcripts in individual cells allows analysis of polygenic activity such as the simultaneous transcription of more than one member of the var multigene family in Plasmodium falciparum infected erythrocytes ¹. The technique is adaptable and can be used on different types of genes, cells and organisms.

Смотреть дополнительные видео

Single cell Gene Transcription

RNA Fluorescent In Situ Hybridization FISH

Plasmodium Falciparum Infected Erythrocytes

PfEMP1 Protein Variants

Var Genes

Mutually Exclusive Regulation

Transcription Assays

RNA FISH Methodology

Digoxigenin labeled Antisense RNA Probes

Biotin labeled Antisense RNA Probes

TSA Plus Fluorescence Palette System