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Mass Spectrometric Analysis of Glycosphingolipid Antigens

16.5K Views

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13:09 min

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April 16th, 2013

DOI :

10.3791/4224-v

April 16th, 2013

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1Undergraduate Program, Rice University , 2Proteomics Facility, Department of Pathology, University of Texas MD Anderson Cancer Center , 3Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center , 4University of Texas Graduate School of Biological Sciences at Houston

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Mass Spectrometric Analysis
Glycosphingolipid Antigens
GSL s
Glycoconjugate Biomacromolecules
Structural Information
Embryonic Development
Signal Transduction
Immune Receptor Recognition
Sugar Moieties
Isomers
Lipid Moieties
Fatty Acyl Chain Length
Unsaturation
Hydroxylation
Carbohydrate Portion
Ceramide Portion
Tri hexosylceramides
Globotriaosylceramide Gal 4Gal 4Glc 1Cer
Isoglobotriaosylceramide Gal 3Gal 4Glc 1Cer
Molecular Masses
Sugar Linkages
Biological Functions
Modification Of Ceramide Part
Alpha galactosylceramide
Cytokine Secretion Profiles
Invariant NKT Cells
Cancer Models
Autoimmune Diseases
Structural Analysis Of Isomers
Immune Organs And Cells

Главы в этом видео

0:05

Title

1:47

Extraction of Glycosphingolipids from Rat Leukemia Cells

2:50

Separation of Neutral and Acidic Lipids

5:03

Removal of Phospholipids from the Neutral Fraction

8:14

Permethylation of Glycosphingolipids

10:04

Results: Mass Spectra of Permethylated Glycosphingolipids

12:12

Conclusion

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